The Journal of Immunology, Vol 143, Issue 3 984-988, Copyright © 1989 by American Association of Immunologists

ARTICLES

Characterization of B lymphocytes present in the demyelinating lesions induced by Theiler's virus [published erratum appears in J Immunol 1989 Sep 15;143(6):2081]

E Cash, A Bandeira, S Chirinian and M Brahic
Laboratory of Slow Viruses, UA CNRS 1157, Paris, France.

Theiler's virus, a murine picornavirus, persists in the central nervous system of SJL/J mice and causes inflammation and demyelination in the white matter of spinal cord. We isolated inflammatory cells from the central nervous system of infected animals and studied their functions in vitro. Flow microfluorimetry analysis showed the presence of all major lymphocyte subsets, namely CD4+ and CD8+ T cells as well as B lymphocytes. B lymphocytes were activated in vitro and the antigenic specificity of secreted Ig was determined by immunoblotting. Secreted Ig reacted strongly with viral capsid proteins VP1 and VP2 and had neutralizing activity. They reacted also with two nonviral white matter components which were present only in infected animals. Therefore, it is likely that Igs secreted at the site of infection play a role in limiting virus spread. It is also possible that virus induced autoreactive antibodies participate in demyelination.

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