| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 143, Issue 3 939-944, Copyright © 1989 by American Association of Immunologists
ARTICLES |
AP Sherblom, N Sathyamoorthy, JM Decker and AV Muchmore
Department of Biochemistry, University of Maine, Orono 04469.
Utilizing a solid phase binding assay, we have demonstrated that rIL-2 binds with high affinity to the human urinary glycoprotein uromodulin. This binding is specifically inhibited by the saccharides diacetylchitobiose and Man(alpha 1-3)(Man(alpha 1-6]Man-O-methyl and by the high mannose glycopeptides Man5GlcNAc2-R and Man6GlcNAc2-R, but not by Man9GlcNAc2-R. rIL-2 also binds OVA, a glycoprotein which contains approximately 50% high mannose chains at a single glycosylation site, and to yeast mannan. This binding is inhibited by the same battery of saccharides which inhibit the binding to uromodulin. The conclusion that rIL-2 is a lectin is further supported by the observation that the sequence of IL-2 shares 27% homology with a 33-residue sequence of the carbohydrate-binding domain of human mannose-binding protein. The potential physiologic relevance of the carbohydrate binding activity is further elucidated by studies which show that 1) binding of soluble rIL- 2 to immobilized uromodulin is enhanced at a pH of 4 to5 in the presence of divalent cations, and 2) neither uromodulin nor the high mannose glycopeptide Man5GlcNAc2Asn blocks the binding of rIL-2 to the IL-2R. Thus the carbohydrate-binding site of rIL-2 is distinct from the cell surface receptor-binding site, and might function preferentially in acidic microenvironments.
This article has been cited by other articles:
|
|
 |
|
  G. Roberts, E. Tarelli, K. A. Homer, J. Philpott-Howard, and D. Beighton Production of an Endo-beta -N-Acetylglucosaminidase Activity Mediates Growth of Enterococcus faecalis on a High-Mannose-Type Glycoprotein J. Bacteriol., February 15, 2000; 182(4): 882 - 890. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Fukushima, S. Hara-Kuge, T. Ohkura, A. Seko, H. Ideo, T. Inazu, and K. Yamashita Lectin-like Characteristics of Recombinant Human Interleukin-1beta Recognizing Glycans of the Glycosylphosphatidylinositol Anchor J. Biol. Chem., April 18, 1997; 272(16): 10579 - 10584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Easton, M. S. Patankar, G. F. Clark, H. R. Morris, and A. Dell Pregnancy-associated Changes in the Glycosylation of Tamm-Horsfall Glycoprotein. EXPRESSION OF SIALYL LEWISx SEQUENCES ON CORE 2 TYPE O-GLYCANS DERIVED FROM UROMODULIN J. Biol. Chem., July 14, 2000; 275(29): 21928 - 21938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cebo, T. Dambrouck, E. Maes, C. Laden, G. Strecker, J.-C. Michalski, and J.-P. Zanetta Recombinant Human Interleukins IL-1alpha , IL-1beta , IL-4, IL-6, and IL-7 Show Different and Specific Calcium-independent Carbohydrate-binding Properties J. Biol. Chem., February 16, 2001; 276(8): 5685 - 5691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukushima and K. Yamashita Interleukin-2 Carbohydrate Recognition Modulates CTLL-2 Cell Proliferation J. Biol. Chem., March 2, 2001; 276(10): 7351 - 7356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fukushima, S. Hara-Kuge, H. Ideo, and K. Yamashita Carbohydrate Recognition Site of Interleukin-2 in Relation to Cell Proliferation J. Biol. Chem., August 10, 2001; 276(33): 31202 - 31208. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
