The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mizutani, H.
Right arrow Articles by Kupper, T. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mizutani, H.
Right arrow Articles by Kupper, T. S.

The Journal of Immunology, Vol 143, Issue 3 896-901, Copyright © 1989 by American Association of Immunologists

ARTICLES

Synergistic interactions of IL-1 and IL-6 in T cell activation. Mitogen but not antigen receptor-induced proliferation of a cloned T helper cell line is enhanced by exogenous IL-6

H Mizutani, LT May, PB Sehgal and TS Kupper
Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520.

Recent reports have indicated that in addition to its well characterized effects on B cells and hepatocytes. IL-6 also affects murine and human T cells and thymocytes. Our study was designed to analyze the effects of IL-1 and IL-6 in providing a second signal in T cell activation in the D10.G4.1 assay. Highly purified human rIL-6 was tested. rIL-6 had modest but detectable activity in the D10.G4.1 assay. Maximal enhancement of proliferation induced by IL-6 in the presence of mitogen in the D10.G4.1 assay was always far less than that induced by IL-1. The D10.G4.1 assay was used to test the possibility of synergistic interactions between IL-1 and IL-6. Quantities of IL-6 which alone were not costimulatory to D10 cells enhanced IL-1-induced D10 proliferation significantly when Con A was used as a costimulus. This synergistic response could be partially blocked by antibodies to rIL-6 and fully blocked by a mAb to rIL-1 alpha. In contrast, when 3D3 (a clonotypic activating anti-TCR mAb) was used as a costimulus, no synergistic interaction between IL-1 and IL-6 could be detected. The proliferation of D10 cells induced by IL-1 and 3D3 was unaffected by antibodies to IL-6 but was completely neutralized by antibodies to IL- 1. These data suggest that although IL-6 alone cannot substitute for IL- 1 in functional assays for IL-1, the presence of IL-6 significantly enhances T cell responses to IL-1 in the context of the appropriate costimulatory signal. These observations have important implications regarding the specificity and utility of the D10.G4.1 assay for the measurement of IL-1 in biologic samples.


This article has been cited by other articles:


Home page
 CVIHome page
S. T. Azar, H. Tamim, H. N. Beyhum, M. Z. Habbal, and W. Y. Almawi
Type I (Insulin-Dependent) Diabetes Is a Th1- and Th2-Mediated Autoimmune Disease
Clin. Vaccine Immunol., May 1, 1999; 6(3): 306 - 310.
[Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
W. Y. Almawi, H. Tamim, and S. T. Azar
T Helper Type 1 and 2 Cytokines Mediate the Onset and Progression of Type I (Insulin-Dependent) Diabetes
J. Clin. Endocrinol. Metab., May 1, 1999; 84(5): 1497 - 1502.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
R. L. Chelvarajan, N. L. Gilbert, and S. Bondada
Neonatal Murine B Lymphocytes Respond to Polysaccharide Antigens in the Presence of IL-1 and IL-6
J. Immunol., October 1, 1998; 161(7): 3315 - 3324.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.