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The Journal of Immunology, Vol 143, Issue 3 858-863, Copyright © 1989 by American Association of Immunologists

ARTICLES

CD8+CD4- lymphocyte lines can harbor the AIDS virus in vitro

H Tsubota, DJ Ringler, M Kannagi, NW King, KR Solomon, JJ MacKey, DG Walsh and NL Letvin
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.

A detailed definition of AIDS virus-specific T lymphocytes will require the generation and characterization of HIV-1-specific, cloned T cell populations. In our studies, we show that CD8+CD4- lymphocyte lines, derived from PBL of rhesus monkeys infected with simian immunodeficiency virus of macaques and humans infected with HIV-1, can harbor AIDS viruses. CD8+CD4- lymphocyte lines derived from infected individuals are shown to express AIDS virus-encoded proteins and generate reverse transcriptase activity. Infection of these CD8+CD4- lymphocytes is confirmed at the single cell level by the techniques of immunoelectronmicroscopy and two-color immunohistochemistry. This observation suggests that it may prove problematic to generate cloned, functional T lymphocyte populations from AIDS virus-infected individuals and raises the possibility that CD8+CD4- cells may serve as reservoirs for the AIDS viruses.


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L. Flamand, R. W. Crowley, P. Lusso, S. Colombini-Hatch, D. M. Margolis, and R. C. Gallo
Activation of CD8+ T lymphocytes through the T cell receptor turns on CD4 gene expression: Implications for HIV pathogenesis
PNAS, March 17, 1998; 95(6): 3111 - 3116.
[Abstract] [Full Text] [PDF]


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