The Journal of Immunology, Vol 143, Issue 12 4069-4077, Copyright © 1989 by American Association of Immunologists

ARTICLES

Assembly, intracellular processing, and expression at the cell surface of the human alpha beta T cell receptor/CD3 complex. Function of the CD3-zeta chain

C Geisler, J Kuhlmann and B Rubin
Tissue Typing Laboratory, State University Hospital, Copenhagen, Denmark.

The TCR/CD3 complex is a multimeric protein complex composed of a minimum of seven transmembrane chains (TCR alpha beta-CD3 gamma delta epsilon zeta 2). Whereas earlier studies have demonstrated that both the TCR-alpha and -beta chains are required for the cell surface expression of the TCR/CD3 complex, the role of the CD3 chains for the TCR/CD3 expression have not been experimentally addressed in human T cells. In this study the function of the CD3-zeta chain for the assembly, intracellular processing, and expression of the TCR/CD3 complex in the human leukemic T cell line Jurkat was investigated. The results indicate that: 1) CD3-zeta is required for the cell surface expression of the TCR/CD3 complex; 2) the pentameric form (TCR alpha beta-CD3 gamma delta epsilon) of the TCR/CD3 complex and single TCR chains associated with CD3 (TCR alpha-CD3 gamma delta epsilon and TCR beta-CD3 gamma delta epsilon) are produced in the endoplasmic reticulum in the absence of CD3-zeta; 3) the CD3-zeta does not associate with TCR alpha-CD3 gamma delta epsilon or TCR beta-CD3 gamma delta epsilon complexes; 4) CD3-zeta associate with the pentameric form of the TCR/CD3 complex in the endoplasmic reticulum to form the heptameric complex (TCR alpha beta-CD3 gamma delta epsilon----TCR alpha beta-CD3 gamma delta epsilon 2); and 5) CD3-zeta is required for the export of the TCR/CD3 complex from the endoplasmic reticulum to the Golgi apparatus for subsequent processing.

This article has been cited by other articles:

				J. L. Roberts, J. P. H. Lauritsen, M. Cooney, R. E. Parrott, E. O. Sajaroff, C. M. Win, M. D. Keller, J. H. Carpenter, J. Carabana, M. S. Krangel, et al. T-B+NK+ severe combined immunodeficiency caused by complete deficiency of the CD3{zeta} subunit of the T-cell antigen receptor complex Blood, April 15, 2007; 109(8): 3198 - 3206. [Abstract] [Full Text] [PDF]

				K. Tsuzaka, Y. Setoyama, K. Yoshimoto, K. Shiraishi, K. Suzuki, T. Abe, and T. Takeuchi A Splice Variant of the TCR {zeta} mRNA Lacking Exon 7 Leads to the Down-Regulation of TCR {zeta}, the TCR/CD3 Complex, and IL-2 Production in Systemic Lupus Erythematosus T Cells J. Immunol., March 15, 2005; 174(6): 3518 - 3525. [Abstract] [Full Text] [PDF]

				B. D. Wines, H. M. Trist, R. C. Monteiro, C. van Kooten, and P. M. Hogarth Fc Receptor {gamma} Chain Residues at the Interface of the Cytoplasmic and Transmembrane Domains Affect Association with Fc{alpha}RI, Surface Expression, and Function J. Biol. Chem., June 18, 2004; 279(25): 26339 - 26345. [Abstract] [Full Text] [PDF]

				A.-M. Steff, S. Trop, M. Maira, J. Drouin, and P. Hugo Opposite Ability of Pre-TCR and {{alpha}}{{beta}}TCR to Induce Apoptosis J. Immunol., April 15, 2001; 166(8): 5044 - 5050. [Abstract] [Full Text] [PDF]

				S. Trop, J. Charron, C. Arguin, and P. Hugo Thymic Selection Generates T Cells Expressing Self-Reactive TCRs in the Absence of CD45 J. Immunol., September 15, 2000; 165(6): 3073 - 3079. [Abstract] [Full Text] [PDF]

				F. R. DeLeo, J. B. Burritt, L. Yu, A. J. Jesaitis, M. C. Dinauer, and W. M. Nauseef Processing and Maturation of Flavocytochrome b558 Include Incorporation of Heme as a Prerequisite for Heterodimer Assembly J. Biol. Chem., April 28, 2000; 275(18): 13986 - 13993. [Abstract] [Full Text] [PDF]

				E. P. G. Martin, J. Arnaud, L. Alibaud, C. Gouaillard, R. Llobera, A. Huchenq-Champagne, and B. Rubin Molecular mechanisms in the TCR (TCR{alpha}{beta}–CD3{delta}{epsilon},{gamma}{epsilon}) interaction with {zeta}2 homodimers: clues from a `phenotypic revertant' clone Int. Immunol., July 1, 1999; 11(7): 1005 - 1015. [Abstract] [Full Text] [PDF]