The Journal of Immunology, Vol 142, Issue 6 2081-2088, Copyright © 1989 by American Association of Immunologists

ARTICLES

Selective expression of class II E alpha d gene in transgenic mice

LC Burkly, D Lo, C Cowing, RD Palmiter, RL Brinster and RA Flavell
Biogen Research Corporation, Cambridge, MA 02142.

Class II genes of the MHC must be expressed by APC for activation of CD4+ T cells and efficient delivery of T cell help to B lymphocytes. Class II genes have restricted tissue expression and are under complex regulation. By using various deletion constructs of the class II E alpha d gene in transgenic mice we have mapped different 5' flanking regions which control E alpha d gene expression in distinct cell types. We demonstrate dissociate expression of E alpha d within the macrophage lineage as well as within the B cell lineage, and present evidence for a repressive element operative in B cells and macrophages. We describe the generation of novel transgenic lines with limited constitutive and inducible E alpha mRNA and I-E protein.

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