The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gilkeson, G. S.
Right arrow Articles by Pisetsky, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gilkeson, G. S.
Right arrow Articles by Pisetsky, D. S.

The Journal of Immunology, Vol 142, Issue 5 1482-1486, Copyright © 1989 by American Association of Immunologists

ARTICLES

Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA

GS Gilkeson, JP Grudier, DG Karounos and DS Pisetsky
Medical Research Service, Durham Veterans Administration Hospital, North Carolina 27710.

Because of recent observations suggesting that bacterial DNA is immunogenic, the induction in normal mice of antibodies to Escherichia coli (EC) dsDNA was investigated. BALB/c and C57BL/6 mice were immunized with dsEC or ds calf thymus (CT) DNA complexed to methylated BSA in adjuvant; antibody responses were measured by ELISA. In both strains, dsEC DNA immunization induced a much higher anti-dsDNA response to dsEC DNA than did dsCT DNA immunization. Neither immunized group showed an appreciable antibody response when tested on dsCT DNA. Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag. These results suggest that bacterial dsDNA is immunogenic and that at least some anti-dsDNA specificities can arise by immunization.


This article has been cited by other articles:


Home page
 LupusHome page
K.A.M. Kuruma, E.F. Borba, M.H. Lopes, J.F. de Carvalho, and E. Bonfa
Safety and efficacy of hepatitis B vaccine in systemic lupus erythematosus
Lupus, May 1, 2007; 16(5): 350 - 354.
[Abstract] [PDF]

Home page
 Rheumatology (Oxford)Home page
M. Blank and Y. Shoenfeld
Experimental models of systemic lupus erythematosus: anti-dsDNA in murine lupus
Rheumatology, September 1, 2005; 44(9): 1086 - 1089.
[Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
G. Riemekasten and B. H. Hahn
Key autoantigens in SLE
Rheumatology, August 1, 2005; 44(8): 975 - 982.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Brummel and P. Lenert
Activation of Marginal Zone B Cells from Lupus Mice with Type A(D) CpG-Oligodeoxynucleotides
J. Immunol., February 15, 2005; 174(4): 2429 - 2434.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Koarada, Y. Wu, N. Fertig, D. A. Sass, M. Nalesnik, J. A. Todd, P. A. Lyons, J. Fenyk-Melody, D. B. Rainbow, L. S. Wicker, et al.
Genetic Control of Autoimmunity: Protection from Diabetes, but Spontaneous Autoimmune Biliary Disease in a Nonobese Diabetic Congenic Strain
J. Immunol., August 15, 2004; 173(4): 2315 - 2323.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
K Hasegawa and T Hayashi
Synthetic CpG oligodeoxynucleotides accelerate the development of lupus nephritis during preactive phase in NZB xNZWF1 mice
Lupus, November 1, 2003; 12(11): 838 - 845.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
I. Gursel, M. Gursel, H. Yamada, K. J. Ishii, F. Takeshita, and D. M. Klinman
Repetitive Elements in Mammalian Telomeres Suppress Bacterial DNA-Induced Immune Activation
J. Immunol., August 1, 2003; 171(3): 1393 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
R. A. Zeuner, D. M. Klinman, G. Illei, C. Yarboro, K. J. Ishii, M. Gursel, and D. Verthelyi
Response of peripheral blood mononuclear cells from lupus patients to stimulation by CpG oligodeoxynucleotides
Rheumatology, April 1, 2003; 42(4): 563 - 569.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
B Deocharan, X Qing, E Beger, and C Putterman
Antigenic triggers and molecular targets for anti-double-stranded DNA antibodies
Lupus, December 1, 2002; 11(12): 865 - 871.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
H. Yamada, I. Gursel, F. Takeshita, J. Conover, K. J. Ishii, M. Gursel, S. Takeshita, and D. M. Klinman
Effect of Suppressive DNA on CpG-Induced Immune Activation
J. Immunol., November 15, 2002; 169(10): 5590 - 5594.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Sekine, C. M. Reilly, I. D. Molano, G. Garnier, A. Circolo, P. Ruiz, V. M. Holers, S. A. Boackle, and G. S. Gilkeson
Complement Component C3 Is Not Required for Full Expression of Immune Complex Glomerulonephritis in MRL/lpr Mice
J. Immunol., May 15, 2001; 166(10): 6444 - 6451.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
Y. Sun, K.-Y. Fong, M. C. M. Chung, and Z.-J. Yao
Peptide mimicking antigenic and immunogenic epitope of double-stranded DNA in systemic lupus erythematosus
Int. Immunol., February 1, 2001; 13(2): 223 - 232.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Watanabe, G. Garnier, A. Circolo, R. A. Wetsel, P. Ruiz, V. M. Holers, S. A. Boackle, H. R. Colten, and G. S. Gilkeson
Modulation of Renal Disease in MRL/lpr Mice Genetically Deficient in the Alternative Complement Pathway Factor B
J. Immunol., January 15, 2000; 164(2): 786 - 794.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. M. Klinman, J. Conover, and C. Coban
Repeated Administration of Synthetic Oligodeoxynucleotides Expressing CpG Motifs Provides Long-Term Protection against Bacterial Infection
Infect. Immun., November 1, 1999; 67(11): 5658 - 5663.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. S. Gilkeson, J. Conover, M. Halpern, D. S. Pisetsky, A. Feagin, and D. M. Klinman
Effects of Bacterial DNA on Cytokine Production by (NZB/NZW)F1 Mice
J. Immunol., October 15, 1998; 161(8): 3890 - 3895.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
H. B. Richards, M. Satoh, M. Shaw, C. Libert, V. Poli, and W. H. Reeves
Interleukin 6 Dependence of Anti-DNA Antibody Production: Evidence for Two Pathways of Autoantibody Formation in Pristane-induced Lupus
J. Exp. Med., September 7, 1998; 188(5): 985 - 990.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A.-K. Yi, R. Tuetken, T. Redford, M. Waldschmidt, J. Kirsch, and A. M. Krieg
CpG Motifs in Bacterial DNA Activate Leukocytes Through the pH-Dependent Generation of Reactive Oxygen Species
J. Immunol., May 15, 1998; 160(10): 4755 - 4761.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
D. S. Pisetsky
Antibody Responses to DNA in Normal Immunity and Aberrant Immunity
Clin. Vaccine Immunol., January 1, 1998; 5(1): 1 - 6.
[Full Text]

Home page
 LupusHome page
D.A. Isenberg, C.T. Ravirajan, A. Rahman, and J. Kalsi
The role of antibodies to DNA in systemic lupus erythematosus A review and introduction to an international workshop on DNA antibodies held in London, May 1996
Lupus, January 1, 1997; 6(3): 290 - 304.
[PDF]

Home page
 LupusHome page
J. George and Y. Shoenfeld
Infections, idiotypes and SLE
Lupus, October 1, 1995; 4(5): 333 - 335.
[PDF]

Home page
 LupusHome page
R. Suenaga and N. I. Abdou
Lupus-Derived Human Monoclonal IgM anti-DNA Antibody Displays Monospecificity, High Affinity and Private Idiotype Specificity
Lupus, December 1, 1992; 1(6): 363 - 368.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.