The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parker, K. P.
Right arrow Articles by Kilian, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parker, K. P.
Right arrow Articles by Kilian, P. L.
Right arrowPubmed/NCBI databases
*Substance via MeSH

The Journal of Immunology, Vol 142, Issue 2 537-542, Copyright © 1989 by American Association of Immunologists

ARTICLES

Presence of IL-1 receptors on human and murine neutrophils. Relevance to IL-1-mediated effects in inflammation

KP Parker, WR Benjamin, KL Kaffka and PL Kilian
Department of Immunopharmacology, Hoffmann-LaRoche, Inc., Nutley, NJ 07110.

Inflammatory responses are characterized by the infiltration of polymorphonuclear neutrophils (PMN) at the involved site. IL-1 may have an important role in mediating this response, but whether IL-1 acts directly on PMN is controversial. In this study, we examined PMN for the presence of IL-1R and determined the effect of IL-1 on PMN migration in vivo. Thioglycollate, proteose-peptone, or IL-1 elicited peritoneal exudate cells were found to bind 125I-IL-1 alpha in a specific and saturable manner. This binding was localized to the PMN in the exudate. Scatchard plot analysis indicates the presence of approximately 1700 receptors per PMN and an apparent dissociation constant of 3.0 x 10(-10) M. Binding sites for 125I-IL-1 alpha were also found on human PMN prepared from peripheral blood. There are approximately 900 receptors per cell on human PMN with a dissociation constant similar to that observed for elicited murine PMN. Binding of 125I-IL-1 alpha to the mouse and human PMN is inhibited by both recombinant human IL-1 alpha and IL-1 beta, indicating that both IL-1 proteins bind to the same receptor on these cells. Human PMN were able to internalize radioiodinated IL-1. We conclude that PMN possess receptors for IL-1 and that these binding sites may be important in mediating IL-1 effects on granulocytes that are involved in the inflammatory response.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
A. L. Patrick, J. Rullo, S. Beaudin, P. Liaw, and A. E. Fox-Robichaud
Hepatic leukocyte recruitment in response to time-limited expression of TNF-{alpha} and IL-1beta
Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G663 - G672.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
S. Miyazaki, F. Ishikawa, T. Fujikawa, S. Nagata, and K. Yamaguchi
Intraperitoneal Injection of Lipopolysaccharide Induces Dynamic Migration of Gr-1high Polymorphonuclear Neutrophils in the Murine Abdominal Cavity
Clin. Vaccine Immunol., May 1, 2004; 11(3): 452 - 457.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. SHIMIZU, K. DOBASHI, K. IIZUKA, T. HORIE, K. SUZUKI, H. TUKAGOSHI, T. NAKAZAWA, Y. NAKAZATO, and M. MORI
Contribution of Small GTPase Rho and Its Target Protein ROCK in a Murine Model of Lung Fibrosis
Am. J. Respir. Crit. Care Med., January 1, 2001; 163(1): 210 - 217.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.