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The Journal of Immunology, Vol 142, Issue 1 179-184, Copyright © 1989 by American Association of Immunologists

ARTICLES

Identification and localization of allergenic determinants on grass group I antigens using monoclonal antibodies

RE Esch and DG Klapper
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599.

Epitopes recognized by five mAb which block the binding of human IgE antibodies to grass group I (GpI) Ag were characterized and partially mapped. Site specificity studies defined four apparently non- overlapping blocking antibody binding sites on the meadow fescue GpI molecule, Fes e I. One of these sites (site A) was localized to a 14,000 m.w. fragment designated P3 generated by CNBr cleavage of purified Fes e I. The P3 peptide possessed human IgE binding sites as well as other epitopes (non-site A) defined by 19 other anti-GpI mAb. All of the P3 reactive antibodies recognized cross-reactive determinants found on GpI Ag isolated from five different grasses suggesting that P3 is a conserved portion of grass GpI molecules. The P3 fragment from Fes e I was used to immunize mice and induced antibodies which reacted with intact GpI Ag from all 5 different grasses currently being studied in this laboratory.


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