The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rennick, D.
Right arrow Articles by Yang, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rennick, D.
Right arrow Articles by Yang, G.

The Journal of Immunology, Vol 142, Issue 1 161-166, Copyright © 1989 by American Association of Immunologists

ARTICLES

IL-3 and stromal cell-derived factor synergistically stimulate the growth of pre-B cell lines cloned from long-term lymphoid bone marrow cultures

D Rennick, J Jackson, C Moulds, F Lee and G Yang
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304.

The addition of IL-3 to modified Whitlock-Witte long-term lymphocyte cultures was found to enhance the growth of a small but significant number of B cell precursors supported by an adherent stromal cell monolayer. Several pre-B cell lines were cloned from IL-3-treated long- term lymphocyte cultures. The growth requirements and physical properties of one representative clone, BL/3, are described. BL/3 cells were shown to be unresponsive to IL-3 except when it is used at very high concentrations. In contrast, significant growth was stimulated by stromal cell conditioned medium previously shown to contain a pre-B cell growth factor. Optimal growth of the pre-B cell clone was stimulated by stromal cell conditioned medium plus IL-3. Synergy between the stromal cell-derived factor and IL-3 occurred when IL-3 was used over a wide range of concentrations including a relatively low amount that was ineffective as a growth stimulus by itself. The finding that more than one factor is required to sustain optimal growth of some pre-B cells parallels the complex growth requirements reported for some primitive myeloid/erythroid progenitors.


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Bihl, M. Tamm, M. Nauck, H. Wieland, A. P. Perruchoud, and M. Roth
Proliferation of Human Non-Small-Cell Lung Cancer Cell Lines: Role of Interleukin-6
Am. J. Respir. Cell Mol. Biol., October 1, 1998; 19(4): 606 - 612.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Ikushima, T. Inukai, T. Inaba, S. D. Nimer, J. L. Cleveland, and A. T. Look
Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes
PNAS, March 18, 1997; 94(6): 2609 - 2614.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
F Hirayama and M Ogawa
Cytokine regulation of early lymphohematopoietic development
Stem Cells, July 1, 1996; 14(4): 369 - 375.
[Abstract]

Home page
 J. Biol. Chem.Home page
M. J. Guimaraes, J. F. Bazan, J. Castagnola, S. Diaz, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, A. Varki, and A. Zlotnik
Molecular Cloning and Characterization of Lysosomal Sialic Acid O-Acetylesterase
J. Biol. Chem., June 7, 1996; 271(23): 13697 - 13705.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1989 by The American Association of Immunologists, Inc. All rights reserved.