| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 142, Issue 1 100-109, Copyright © 1989 by American Association of Immunologists
ARTICLES |
SJ Warner and P Libby
Department of Medicine, Tufts University, Boston, MA 02111.
TNF-alpha (also known as cachectin) may produce many of its important effects in vivo by actions on blood vessels. Endothelial cells are well known to respond to TNF-alpha. We investigated whether vascular smooth muscle cells (SMC), the most abundant cell type in most vessels, also respond to TNF-alpha and the related cytokine lymphotoxin (TNF-beta). Both human rTNF-alpha and beta (0.1 to 100 ng/ml) induced transient accumulation of IL-1 mRNA by adult human vascular SMC that peaked between 1 and 4 h. The inhibitor of RNA synthesis actinomycin D (1 microgram/ml) blocked the induction of IL-1 mRNA, whereas inhibition of protein synthesis with cycloheximide (1 microgram/ml) resulted in a marked "superinduction" of both IL-1 alpha and IL-1 beta mRNA species. TNF-alpha treatment also increased intracellular biologically active IL- 1 and subsequent release of IL-1 activity from SMC. Metabolic labeling and immunoprecipitation with specific antibodies demonstrated de novo synthesis of IL-1 alpha and IL-1 beta precursors in TNF-treated or lymphotoxin-treated SMC. TNF-alpha also activated other SMC functions including the concentration-dependent release of PGE2 from SMC, and time-dependent induction of the gene for (2'-5')-oligoadenylate synthetase, an enzyme thought to mediate the anti-viral and anti- proliferative actions of IFN. We also explored whether SMC, which both produce and respond to IL-1, might also express either of the TNF genes. Bacterial LPS (10 micrograms/ml) caused slight accumulation of TNF-alpha transcripts. Incubation of SMC for 4 h with inhibitors of protein synthesis alone caused little or no elevation of TNF-alpha mRNA, but simultaneous addition of LPS ("superinduction" conditions) induced large amounts of TNF-alpha (but not TNF-beta) mRNA. Cells treated with anisomycin (1 microgram/ml) and LPS, then washed to remove this reversible inhibitor of protein synthesis, released TNF-alpha into the medium, as assessed by the L929 cytotoxicity assay and by metabolic labeling and immunoprecipitation. Thus, SMC both respond to both TNF and lymphotoxin and can produce TNF-alpha, a cytokine with numerous effects on vascular cells of potential significance in the pathophysiology of septic shock and other inflammatory conditions.
This article has been cited by other articles:
|
|
 |
|
  H. Loppnow, K. Werdan, and M. Buerke Invited review: Vascular cells contribute to atherosclerosis by cytokine- and innate-immunity-related inflammatory mechanisms Innate Immunity, April 1, 2008; 14(2): 63 - 87. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Y. Sung, H. Guan, A. Czibula, A. R. King, K. Eder, E. Heath, S. K. Suvarna, S. K. Dower, A. G. Wilson, S. E. Francis, et al. Human Tribbles-1 Controls Proliferation and Chemotaxis of Smooth Muscle Cells via MAPK Signaling Pathways J. Biol. Chem., June 22, 2007; 282(25): 18379 - 18387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Ferreri Estrogen-TNF interactions and vascular inflammation Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2566 - H2569. [Full Text] [PDF]
|
|
|
|
|
|
D. Bose, K. Leineweber, T. Konorza, A. Zahn, M. Brocker-Preuss, K. Mann, M. Haude, R. Erbel, and G. Heusch Release of TNF-{alpha} during stent implantation into saphenous vein aortocoronary bypass grafts and its relation to plaque extrusion and restenosis Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2295 - H2299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tedgui and Z. Mallat Cytokines in Atherosclerosis: Pathogenic and Regulatory Pathways Physiol Rev, April 1, 2006; 86(2): 515 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Boos, R. A. Anderson, and G. Y.H. Lip Is atrial fibrillation an inflammatory disorder? Eur. Heart J., January 2, 2006; 27(2): 136 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Md. R. Abid, K. Yano, S. Guo, V. I. Patel, G. Shrikhande, K. C. Spokes, C. Ferran, and W. C. Aird Forkhead Transcription Factors Inhibit Vascular Smooth Muscle Cell Proliferation and Neointimal Hyperplasia J. Biol. Chem., August 19, 2005; 280(33): 29864 - 29873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Shai, M. B. Schulze, J. E. Manson, K. M. Rexrode, M. J. Stampfer, C. Mantzoros, and F. B. Hu A Prospective Study of Soluble Tumor Necrosis Factor-{alpha} Receptor II (sTNF-RII) and Risk of Coronary Heart Disease Among Women With Type 2 Diabetes Diabetes Care, June 1, 2005; 28(6): 1376 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Sardo, M. Castaldo, M. Cinquegrani, M. Bonaiuto, L. Fontana, S. Campo, G. M. Campo, D. Altavilla, and A. Saitta Effects of AT1 Receptor Antagonist Losartan on sICAM-1 and TNF-a Levels in Uncomplicated Hypertensive Patients Angiology, March 1, 2004; 55(2): 195 - 203. [Abstract] [PDF]
|
|
|
|
 |
|
 D. A. Goukassian, R. Kishore, K. Krasinski, C. Dolan, C. Luedemann, Y.-s. Yoon, M. Kearney, A. Hanley, H. Ma, T. Asahara, et al. Engineering the Response to Vascular Injury: Divergent Effects of Deregulated E2F1 Expression on Vascular Smooth Muscle Cells and Endothelial Cells Result in Endothelial Recovery and Inhibition of Neointimal Growth Circ. Res., July 25, 2003; 93(2): 162 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Ungvari, A. Csiszar, J. G. Edwards, P. M. Kaminski, M. S. Wolin, G. Kaley, and A. Koller Increased Superoxide Production in Coronary Arteries in Hyperhomocysteinemia: Role of Tumor Necrosis Factor-{alpha}, NAD(P)H Oxidase, and Inducible Nitric Oxide Synthase Arterioscler. Thromb. Vasc. Biol., March 1, 2003; 23(3): 418 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Gerthoffer and C. A. Singer Secretory Functions of Smooth Muscle: Cytokines and Growth Factors Mol. Interv., November 1, 2002; 2(7): 447 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Futterman and L. Lemberg High-Sensitivity C-Reactive Protein Is the Most Effective Prognostic Measurement of Acute Coronary Events Am. J. Crit. Care., September 1, 2002; 11(5): 482 - 486. [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Zimmerman, C. H. Selzman, L. L. Reznikov, S. A. Miller, C. D. Raeburn, J. Emmick, X. Meng, and A. H. Harken Lack of TNF-alpha attenuates intimal hyperplasia after mouse carotid artery injury Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2002; 283(2): R505 - R512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Sakash, G. I. Byrne, A. Lichtman, and P. Libby Cytokines Induce Indoleamine 2,3-Dioxygenase Expression in Human Atheroma-Associated Cells: Implications for Persistent Chlamydophila pneumoniae Infection Infect. Immun., July 1, 2002; 70(7): 3959 - 3961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Schreyer, C. M. Vick, and R. C. LeBoeuf Loss of Lymphotoxin-alpha but Not Tumor Necrosis Factor-alpha Reduces Atherosclerosis in Mice J. Biol. Chem., March 29, 2002; 277(14): 12364 - 12368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-H. Lee, S.-H. Kim, Y. Lee, B. B. Lee, B. Kwon, H. Song, B. S. Kwon, and J.-E. Park Tumor Necrosis Factor Receptor Superfamily 14 Is Involved in Atherogenesis by Inducing Proinflammatory Cytokines and Matrix Metalloproteinases Arterioscler. Thromb. Vasc. Biol., December 1, 2001; 21(12): 2004 - 2010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. O. Nossuli, N. G. Frangogiannis, P. Knuefermann, V. Lakshminarayanan, O. Dewald, A. J. Evans, J. Peschon, D. L. Mann, L. H. Michael, and M. L. Entman Brief murine myocardial I/R induces chemokines in a TNF-alpha -independent manner: role of oxygen radicals Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2549 - H2558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Blake and P. M. Ridker Novel Clinical Markers of Vascular Wall Inflammation Circ. Res., October 26, 2001; 89(9): 763 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sasu, A. L. Cooper, and D. Beasley Juxtacrine effects of IL-1{alpha} precursor promote iNOS expression in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1615 - H1623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Rainger and G. B. Nash Cellular Pathology of Atherosclerosis : Smooth Muscle Cells Prime Cocultured Endothelial Cells for Enhanced Leukocyte Adhesion Circ. Res., March 30, 2001; 88(6): 615 - 622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Rectenwald, L. L. Moldawer, T. S. Huber, J. M. Seeger, and C. K. Ozaki Direct Evidence for Cytokine Involvement in Neointimal Hyperplasia Circulation, October 3, 2000; 102(14): 1697 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Obara, A. Takayanagi, J. Hirahashi, K. Tanaka, G. Wakabayashi, K. Matsumoto, M. Shimazu, N. Shimizu, and M. Kitajima Overexpression of Truncated I{kappa}B{alpha} Induces TNF-{alpha}-Dependent Apoptosis in Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., October 1, 2000; 20(10): 2198 - 2204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Ridker, N. Rifai, M. Pfeffer, F. Sacks, S. Lepage, and E. Braunwald Elevation of Tumor Necrosis Factor-{alpha} and Increased Risk of Recurrent Coronary Events After Myocardial Infarction Circulation, May 9, 2000; 101(18): 2149 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Flak, L. N. Heiss, J. T. Engle, and W. E. Goldman Synergistic Epithelial Responses to Endotoxin and a Naturally Occurring Muramyl Peptide Infect. Immun., March 1, 2000; 68(3): 1235 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. de Frutos, L. S. de Miguel, M. Garcia-Duran, F. Gonzalez-Fernandez, J. A. Rodriguez-Feo, M. Monton, J. Guerra, J. Farre, S. Casado, and A. Lopez-Farre NO from smooth muscle cells decreases NOS expression in endothelial cells: role of TNF-alpha Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1317 - H1325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Cooper and D. Beasley Hypoxia stimulates proliferation and interleukin-1alpha production in human vascular smooth muscle cells Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1326 - H1337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamakawa, S. Eguchi, T. Matsumoto, Y. Yamakawa, K. Numaguchi, I. Miyata, C. M. Reynolds, E. D. Motley, and T. Inagami Intracellular Signaling in Rat Cultured Vascular Smooth Muscle Cells: Roles of Nuclear Factor-{kappa}B and p38 Mitogen-Activated Protein Kinase on Tumor Necrosis Factor-{alpha} Production Endocrinology, August 1, 1999; 140(8): 3562 - 3572. [Abstract] [Full Text]
|
|
|
|
|
|
R. Kranzhofer, J. Schmidt, C. A. H. Pfeiffer, S. Hagl, P. Libby, and W. Kubler Angiotensin Induces Inflammatory Activation of Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1623 - 1629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Selzman, B. D. Shames, L. L. Reznikov, S. A. Miller, X. Meng, H. A. Barton, A. Werman, A. H. Harken, C. A. Dinarello, and A. Banerjee Liposomal Delivery of Purified Inhibitory-{kappa}B{alpha} Inhibits Tumor Necrosis Factor-{alpha}–Induced Human Vascular Smooth Muscle Proliferation Circ. Res., April 30, 1999; 84(8): 867 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Beasley and A. L. Cooper Constitutive expression of interleukin-1alpha precursor promotes human vascular smooth muscle cell proliferation Am J Physiol Heart Circ Physiol, March 1, 1999; 276(3): H901 - H912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Goetze, X.-P. Xi, Y. Kawano, H. Kawano, E. Fleck, W. A. Hsueh, and R. E. Law TNF-{alpha}–Induced Migration of Vascular Smooth Muscle Cells Is MAPK Dependent Hypertension, January 1, 1999; 33(1): 183 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gonzalez-Fernandez, A. Lopez-Farre, J. A. Rodriguez-Feo, J. Farre, J. Guerra, J. Fortes, I. Millas, M. Garcia-Duran, L. Rico, P. Mata, et al. Expression of Inducible Nitric Oxide Synthase After Endothelial Denudation of the Rat Carotid Artery : Role of Platelets Circ. Res., November 30, 1998; 83(11): 1080 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Braun-Dullaeus, M. J. Mann, and V. J. Dzau Cell Cycle Progression : New Therapeutic Target for Vascular Proliferative Disease Circulation, July 7, 1998; 98(1): 82 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Libby, D. Egan, and S. Skarlatos Roles of Infectious Agents in Atherosclerosis and Restenosis: An Assessment of the Evidence and Need for Future Research Circulation, December 2, 1997; 96(11): 4095 - 4103. [Full Text]
|
|
|
|
|
|
Y.-J. Geng, L. E. Henderson, E. B. Levesque, M. Muszynski, and P. Libby Fas Is Expressed in Human Atherosclerotic Intima and Promotes Apoptosis of Cytokine-Primed Human Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2200 - 2208. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. D. Poss and S. Tonegawa Reduced stress defense in heme oxygenase 1-deficient cells PNAS, September 30, 1997; 94(20): 10925 - 10930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jovinge, A. Hultgardh-Nilsson, J. Regnstrom, and J. Nilsson Tumor Necrosis Factor-{alpha} Activates Smooth Muscle Cell Migration in Culture and Is Expressed in the Balloon-Injured Rat Aorta Arterioscler. Thromb. Vasc. Biol., March 1, 1997; 17(3): 490 - 497. [Abstract] [Full Text]
|
|
|
|
|
|
P. Libby and R. N. Mitchell Cytokines Score a Knockout: Harnessing Gene Targeting to Gain Insight Into the Pathogenesis of Myocarditis Circulation, February 4, 1997; 95(3): 551 - 552. [Full Text]
|
|
|
|
|
|
R. Kranzhofer, S. K. Clinton, K. Ishii, S. R. Coughlin, J. W. Fenton, and P. Libby Thrombin Potently Stimulates Cytokine Production in Human Vascular Smooth Muscle Cells but Not in Mononuclear Phagocytes Circ. Res., August 1, 1996; 79(2): 286 - 294. [Abstract] [Full Text]
|
|
|
|
|
|
H. Tanaka, G. Sukhova, D. Schwartz, and P. Libby Proliferating Arterial Smooth Muscle Cells After Balloon Injury Express TNF-{alpha} but Not Interleukin-1 or Basic Fibroblast Growth Factor Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 12 - 18. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-J. Geng, Q. Wu, M. Muszynski, G. K. Hansson, and P. Libby Apoptosis of Vascular Smooth Muscle Cells Induced by In Vitro Stimulation With Interferon-{gamma}, Tumor Necrosis Factor–{alpha}, and Interleukin-1ß Arterioscler. Thromb. Vasc. Biol., January 1, 1996; 16(1): 19 - 27. [Abstract] [Full Text]
|
|
|
|
|
|
B. E. Rolfe, J. H. Campbell, N. J. Smith, M. W. Cheong, and G. R. Campbell T Lymphocytes Affect Smooth Muscle Cell Phenotype and Proliferation Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1204 - 1210. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Basaran, M.M. Basaran, K.F. Babacan, B. Ener, T. Okay, H. Gok, and M. Ozdemir Serum Tumor Necrosis Factor Levels in Acute Myocardial Infarction and Unstable Angina Pectoris Angiology, April 1, 1993; 44(4): 332 - 337. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
