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The Journal of Immunology, Vol 141, Issue 9 3123-3127, Copyright © 1988 by American Association of Immunologists
ARTICLES |
WW Kwok, D Schwarz, BS Nepom, RA Hock, PS Thurtle and GT Nepom
Virginia Mason Research Center, Seattle, WA 98101.
Retroviral vectors with an internal cytomegalovirus major immediate- early gene enhancer/promoter regulating HLA class II gene expression were used to transfer HLA cDNA into human EBV-transformed B- lymphoblastoid cell lines. HLA-DQ2 beta and DQ3.2 beta cDNA were transferred into DQ3.2 and DQ2 homozygous lymphoblastoid cell lines, respectively. Serologic analysis of the infected cell lines with allospecific mAb demonstrated surface expression of these exogenous DQ molecules implying that DQ alpha-chains from DR3, DQ2-positive cells can pair with DQ3.2 beta-chains and, similarly, DQ alpha-chains from DR4, DQ3.2-positive cells can pair with DQ2 beta-chains. Immunoprecipitation of the introduced DQ3.2 beta molecule resulted in co-purification of the allotype-mismatched endogenous DQ2 alpha polypeptide. We also show that vectors with a cytomegalovirus major immediate-early gene enhancer/promoter result in higher levels of expression of the transduced gene compared to previously described HLA vectors with either the SV-40 early enhancer/promoter or the lymphotropic papovavirus-enhanced SV-40 promoter. Although deletion of HLA cDNA did sometimes occur in the process of generating virus- producing clones, the HLA cDNA is stably maintained in virus-producing clones, once it is generated. This retroviral expression system is a highly efficient way to study HLA gene function.
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 H. Reijonen, J. F. Elliott, P. van Endert, and G. Nepom Differential Presentation of Glutamic Acid Decarboxylase 65 (GAD65) T Cell Epitopes Among HLA-DRB1*0401-Positive Individuals J. Immunol., August 1, 1999; 163(3): 1674 - 1681. [Abstract] [Full Text] [PDF]
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R. A. Ettinger, A. W. Liu, G. T. Nepom, and W. W. Kwok Exceptional Stability of the HLA-DQA1*0102/DQB1*0602 {alpha}{beta} Protein Dimer, the Class II MHC Molecule Associated with Protection from Insulin-Dependent Diabetes Mellitus J. Immunol., December 1, 1998; 161(11): 6439 - 6445. [Abstract] [Full Text] [PDF]
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D. G. Doherty, J. E. Penzotti, D. M. Koelle, W. W. Kwok, T. P. Lybrand, S. Masewicz, and G. T. Nepom Structural Basis of Specificity and Degeneracy of T Cell Recognition: Pluriallelic Restriction of T Cell Responses to a Peptide Antigen Involves Both Specific and Promiscuous Interactions Between the T Cell Receptor, Peptide, and HLA-DR J. Immunol., October 1, 1998; 161(7): 3527 - 3535. [Abstract] [Full Text] [PDF]
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