The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Main, E. K.
Right arrow Articles by Lampson, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Main, E. K.
Right arrow Articles by Lampson, L. A.

The Journal of Immunology, Vol 141, Issue 9 2943-2950, Copyright © 1988 by American Association of Immunologists

ARTICLES

IFN-treated neuroblastoma cell lines remain resistant to T cell- mediated allo-killing, and susceptible to non-MHC-restricted cytotoxicity

EK Main, DS Monos and LA Lampson
Children's Cancer Research Center, Children's Hospital, Philadelphia, PA.

Neuroblastoma cell lines can have very low MHC Ag expression. The cell lines are insensitive to allo-killing by primed CTL, but are sensitive to non-MHC-restricted cytotoxicity. IFN-gamma increased class I expression, but the cells remained insensitive to CTL. Susceptibility to nonrestricted effectors was preserved. Class I+ glioma cell lines behaved similarly. The CTL resistance was localized to the recognition phase. Neuroblastoma lines did not form conjugates with primed T cells, but were lysed if they were coupled to the effectors via lectins. The levels of class I expression, and resistance to CTL, were constant over a range of IFN doses. HLA-A,B,C structure and distribution were studied more intensively on one cell line, CHP-100. HLA-A2 and -A3 were present on greater than or equal to 99% of the cells, in a unimodal distribution. After IFN treatment, the levels were similar to B cell controls. In two-dimensional gel electrophoresis, the molecules co- migrated with those of B cell controls. The defect may thus be in accessory proteins that are necessary for T cell recognition or binding, rather than in the structure or distribution of the HLA-A,B,C proteins.


This article has been cited by other articles:


Home page
 BloodHome page
R. F. Rousseau, A. E. Haight, C. Hirschmann-Jax, E. S. Yvon, D. R. Rill, Z. Mei, S. C. Smith, S. Inman, K. Cooper, P. Alcoser, et al.
Local and systemic effects of an allogeneic tumor cell vaccine combining transgenic human lymphotactin with interleukin-2 in patients with advanced or refractory neuroblastoma
Blood, March 1, 2003; 101(5): 1718 - 1726.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
A. Abendroth, A. Simmons, S. Efstathiou, and R. A. Pereira
Infection with an H2 recombinant herpes simplex virus vector results in expression of MHC class I antigens on the surfaces of human neuroblastoma cells in vitro and mouse sensory neurons in vivo
J. Gen. Virol., October 1, 2000; 81(10): 2375 - 2383.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
A. K. Sarkar and J. G. Nuchtern
Lysis of MYCN-amplified Neuroblastoma Cells by MYCN Peptide-specific Cytotoxic T Lymphocytes
Cancer Res., April 1, 2000; 60(7): 1908 - 1913.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1988 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1988 by The American Association of Immunologists, Inc. All rights reserved.