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The Journal of Immunology, Vol 141, Issue 9 2929-2934, Copyright © 1988 by American Association of Immunologists

ARTICLES

Protein kinase C modulates actin conformation in human T lymphocytes

PD Phatak, CH Packman and MA Lichtman
Department of Medicine, University of Rochester School of Medicine and Dentistry, NY 14642.

We studied the effect of activators and inhibitors of protein kinase C on actin conformation in human blood lymphocytes by flow cytometry and gel electrophoresis. PMA, 1-oleyl-2-acetyl-glycerol, and mezerein, activators of protein kinase C, caused an increase in lymphocyte F- actin within 2 to 5 min. After stimulation with PMA, lymphocytes formed pseudopods containing an increased concentration of F-actin and had an increase of actin in the Triton-insoluble cytoskeletal fraction. Sphingosine and H-7, inhibitors of protein kinase C activation, inhibited the increase in F-actin induced by PMA. The increase in F- actin in response to PMA was striking in Th and Ts lymphocytes (2- to 3- fold increase), but B lymphocytes had only a slight increase (1.15- fold). Thus, activation of protein kinase C modulates actin conformation specifically in T lymphocytes.


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