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The Journal of Immunology, Vol 141, Issue 8 2684-2687, Copyright © 1988 by American Association of Immunologists

ARTICLES

Identification of mouse J chain in human IgM produced by an electrofusion-derived mouse-human heterohybridoma

YG Meng and J Trawinski
Cutter Biological, Division of Miles, Inc., Berkeley, CA 94710.

EBV transformed human B cell line secreting IgM was fused with X63- Ag8.653 mouse cell line. Hybrids were selected in medium containing HAT and ouabain. IgM from a clone was purified by affinity chromatography. Western blot analysis of the heterohybridoma IgM showed the presence of mouse J chain when probed with antiserum against mouse J chain.




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