| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 141, Issue 8 2656-2660, Copyright © 1988 by American Association of Immunologists
ARTICLES |
DC Altieri and TS Edgington
Department of Immunology, Scripps Clinic, La Jolla, CA 92037.
CD11b/CD18 (Mac-1) is a member of the leukocyte integrin family, a group of receptors that have been implicated in various effector functions and cellular collaboration in the immune response. It has been shown previously that CD11b/CD18 on cells of monocyte and myeloid lineage appears to undergo rapid activation and acquire new functional receptor specificities after exposure to selected agonists such as adenosine diphosphate (ADP). We now show that ADP induces a reconformation of the CD11b/CD18 receptor with exposure of new epitopes characteristics of this activated state. By direct binding studies, flow cytometry, and immunoprecipitation experiments, it has been found that the mAb 7E3 reacts with CD11b/CD18 only after ADP-stimulation of the cell suspension. The activated state of CD11b/CD18 induced by ADP and recognized by 7E3 can also be recapitulated by agonists inducing transients in cytosolic Ca2+ such as the chemoattractant FMLP. Moreover, this process of receptor activation does not involve quantitative mobilization of the subcellular storage pool of CD11b/CD18 to the plasma membrane. Because 7E3 also recognizes a qualitative, ADP- mediated activated state of the platelet adhesion receptor GP IIb/IIIa, it is suggested that transients in cytosolic Ca2+ might represent early secondary events for a general pathway of rapid activation of integrin receptors and, as such, represent important signals for cellular interactions in the immune response.
This article has been cited by other articles:
|
|
 |
|
  B. Salanova, M. Choi, S. Rolle, M. Wellner, F. C. Luft, and R. Kettritz beta2-Integrins and Acquired Glycoprotein IIb/IIIa (GPIIb/IIIa) Receptors Cooperate in NF-{kappa}B Activation of Human Neutrophils J. Biol. Chem., September 21, 2007; 282(38): 27960 - 27969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Van de Walle, K. Vanhoorelbeke, Z. Majer, E. Illyes, J. Baert, I. Pareyn, and H. Deckmyn Two Functional Active Conformations of the Integrin {alpha}2{beta}1, Depending on Activation Condition and Cell Type J. Biol. Chem., November 4, 2005; 280(44): 36873 - 36882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Steiner, D. Seidinger, K. Huber, C. Kaun, E. Minar, and C. W. Kopp Effect of Glycoprotein IIb/IIIa Antagonist Abciximab on Monocyte-Platelet Aggregates and Tissue Factor Expression Arterioscler Thromb Vasc Biol, September 1, 2003; 23(9): 1697 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Inoue, T. Uchida, I. Yaguchi, Y. Sakai, K. Takayanagi, and S. Morooka Stent-Induced Expression and Activation of the Leukocyte Integrin Mac-1 Is Associated With Neointimal Thickening and Restenosis Circulation, April 8, 2003; 107(13): 1757 - 1763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Burns, C. W. Smith, and D. C. Walker Unique Structural Features That Influence Neutrophil Emigration Into the Lung Physiol Rev, April 1, 2003; 83(2): 309 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Ustinov and E. F. Plow Delineation of the Key Amino Acids Involved in Neutrophil Inhibitory Factor Binding to the I-domain Supports a Mosaic Model for the Capacity of Integrin alpha Mbeta 2 to Recognize Multiple Ligands J. Biol. Chem., May 17, 2002; 277(21): 18769 - 18776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Pettersen and K. B. Adler Airways Inflammation and COPD* : Epithelial-Neutrophil Interactions Chest, May 1, 2002; 121 (2009): 142S - 150S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Forsyth and H. L. Mathews Lymphocyte Adhesion to Candida albicans Infect. Immun., February 1, 2002; 70(2): 517 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bogousslavsky and J. R. Leclerc Platelet glycoprotein IIb/IIIa antagonists for acute ischemic stroke Neurology, September 1, 2001; 57(90002): S53 - 57. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Corada, F. Liao, M. Lindgren, M. G. Lampugnani, F. Breviario, R. Frank, W. A. Muller, D. J. Hicklin, P. Bohlen, and E. Dejana Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability Blood, March 15, 2001; 97(6): 1679 - 1684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kupatt, H. Habazettl, P. Hanusch, R. Wichels, D. Hahnel, B. F. Becker, and P. Boekstegers c7E3Fab Reduces Postischemic Leukocyte-Thrombocyte Interaction Mediated by Fibrinogen : Implications for Myocardial Reperfusion Injury Arterioscler Thromb Vasc Biol, October 1, 2000; 20(10): 2226 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Wagner and R. A. Roth Neutrophil Migration Mechanisms, with an Emphasis on the Pulmonary Vasculature Pharmacol. Rev., September 1, 2000; 52(3): 349 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Thompson, M. W. Wakelin, K. Y. Larbi, A. Dewar, G. Asimakopoulos, M. A. Horton, M. T. Nakada, and S. Nourshargh Divergent Effects of Platelet-Endothelial Cell Adhesion Molecule-1 and {beta}3 Integrin Blockade on Leukocyte Transmigration In Vivo J. Immunol., July 1, 2000; 165(1): 426 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. i. I. S. Investigators Abciximab in Acute Ischemic Stroke : A Randomized, Double-Blind, Placebo-Controlled, Dose-Escalation Study Stroke, March 1, 2000; 31(3): 601 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Peter, M. Schwarz, C. Conradt, T. Nordt, M. Moser, W. Kubler, and C. Bode Heparin Inhibits Ligand Binding to the Leukocyte Integrin Mac-1 (CD11b/CD18) Circulation, October 5, 1999; 100(14): 1533 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Mickelson, M. N. Ali, N. S. Kleiman, N. M. Lakkis, T. W. Chow, B. J. Hughes, and C. W. Smith Chimeric 7e3 Fab (ReoPro) decreases detectable CD11b on neutrophils from patients undergoing coronary angioplasty J. Am. Coll. Cardiol., January 1, 1999; 33(1): 97 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rogers, E. R. Edelman, and D. I. Simon A mAb to the beta 2-leukocyte integrin Mac-1 (CD11b/CD18) reduces intimal thickening after angioplasty or stent implantation in rabbits PNAS, August 18, 1998; 95(17): 10134 - 10139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Plescia, M. S. Conte, G. VanMeter, G. Ambrosini, and D. C. Altieri Molecular Identification of the Cross-reacting Epitope on alpha Mbeta 2 Integrin I Domain Recognized by Anti-alpha IIbbeta 3 Monoclonal Antibody 7E3 and Its Involvement in Leukocyte Adherence J. Biol. Chem., August 7, 1998; 273(32): 20372 - 20377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Simon, H. Xu, S. Ortlepp, C. Rogers, and N. K. Rao 7E3 Monoclonal Antibody Directed Against the Platelet Glycoprotein IIb/IIIa Cross-reacts With the Leukocyte Integrin Mac-1 and Blocks Adhesion to Fibrinogen and ICAM-1 Arterioscler Thromb Vasc Biol, March 1, 1997; 17(3): 528 - 535. [Abstract] [Full Text]
|
|
|
|
|
|
W. Puzon-McLaughlin, T. A. Yednock, and Y. Takada Regulation of Conformation and Ligand Binding Function of Integrin alpha 5beta 1 by the beta 1 Cytoplasmic Domain J. Biol. Chem., July 12, 1996; 271(28): 16580 - 16585. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bazzoni, D.-T. Shih, C. A. Buck, and M. E. Hemler Monoclonal Antibody 9EG7 Defines a Novel beta(1) Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium J. Biol. Chem., October 27, 1995; 270(43): 25570 - 25577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Crawford and K. Watanabe Cell Adhesion Molecules in Inflammation and Immunity: Relevance to Periodontal Diseases Critical Reviews in Oral Biology & Medicine, January 1, 1994; 5(2): 91 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Sriramarao, M Mendler, and M. Bourdon Endothelial cell attachment and spreading on human tenascin is mediated by alpha 2 beta 1 and alpha v beta 3 integrins J. Cell Sci., January 8, 1993; 105(4): 1001 - 1012. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
