| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 141, Issue 8 2612-2618, Copyright © 1988 by American Association of Immunologists
ARTICLES |
JA Symons, NC Wood, FS Di Giovine and GW Duff
University of Edinburgh, Department of Medicine, U.K.
An ELISA was used to measure soluble IL-2R (sIL-2R) in the sera and synovial fluids of patients with rheumatic diseases. Patients with rheumatoid arthritis had raised levels of sIL-2R both in their sera and in their synovial fluid compared to patients with osteoarthritis and age-matched healthy controls. Mononuclear cells from the synovial fluid of rheumatoid arthritis patients were found to produce spontaneously high levels of sIL-2R which eluted at approximately m.w. 40,000 on gel filtration. In contrast, autologous peripheral blood cells only produced comparable levels upon stimulation with mitogenic lectin. Sequential studies indicated that serum sIL-2R levels were highly correlated with disease activity, indicating that measurement of sIL-2R may be a useful clinical marker in the future. Within the joint, synovial fluid sIL-2R levels correlated significantly with immunoreactive IL-1 beta levels, providing evidence for a role of IL-1 in immune activation in the synovium. In synovial fluids, sIL-2R level also correlated with functional inhibition of IL-2-driven responses in vitro. Furthermore, sIL-2R immunoreactivity in synovial fluid eluted at m.w. 100,000 coeluting with IL-2-inhibitory activity and consistent with a role for sIL-2R in down-regulation of IL-2 responses. The abnormal m.w. may be accounted for by complex formation in the synovial fluid. Given the ability of sIL-2R to bind IL-2, the presence of sIL-2R within the joint may contribute to the well documented "IL-2 defect" seen in rheumatoid arthritis.
This article has been cited by other articles:
|
|
 |
|
  M Cutolo Sex hormone adjuvanting therapy in rheumatoid arthritis Lupus, October 1, 2002; 11(10): 670 - 674. [PDF]
|
|
|
|
 |
|
 B. F. Skinnider and T. W. Mak The role of cytokines in classical Hodgkin lymphoma Blood, May 29, 2002; 99(12): 4283 - 4297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Correia, L. Delgado, J.-C. Roujeau, L. Le Cleach, and J. A. Fleming-Torrinha Soluble Interleukin 2 Receptor and Interleukin 1{alpha} in Toxic Epidermal Necrolysis: A Comparative Analysis of Serum and Blister Fluid Samples Arch Dermatol, January 1, 2002; 138(1): 29 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Akin, L. B. Schwartz, T. Kitoh, H. Obayashi, A. S. Worobec, L. M. Scott, and D. D. Metcalfe Soluble stem cell factor receptor (CD117) and IL-2 receptor alpha chain (CD25) levels in the plasma of patients with mastocytosis: relationships to disease severity and bone marrow pathology Blood, August 15, 2000; 96(4): 1267 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-S. Wang, K.-C. Chow, W.-Y. Li, C.-C. Liu, Y.-C. Wu, and M.-H. Huang Clinical Significance of Serum Soluble Interleukin 2 Receptor-{{alpha}} in Esophageal Squamous Cell Carcinoma Clin. Cancer Res., April 1, 2000; 6(4): 1445 - 1451. [Abstract] [Full Text]
|
|
|
|
|
|
R. Yabkowitz, S. Meyer, D. Yanagihara, D. Brankow, T. Staley, G. Elliott, S. Hu, and B. Ratzkin Regulation of Tie Receptor Expression on Human Endothelial Cells by Protein Kinase C-Mediated Release of Soluble Tie Blood, July 15, 1997; 90(2): 706 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Junghans, A. L. Stone, and M. S. Lewis Biophysical Characterization of a Recombinant Soluble Interleukin 2 Receptor (Tac) J. Biol. Chem., May 3, 1996; 271(18): 10453 - 10460. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. J. Barton, D. K. Blanchard, C. Duan, W. S. Roberts, D. Cavanagh, S. DeCesare, and J. Y. Djeu Interleukin-12 Synergizes With Interleukin-2 to Generate Lymphokine-Activated Killer Activity in Peripheral Blood Mononuclear Cells Cultured in Ovarian Cancer Ascitic Fluid Reproductive Sciences, November 1, 1995; 2(6): 762 - 771. [Abstract] [PDF]
|
|
|
|
|
|
M.N. Manoussakis, G.S. Germanidis, A.A. Drosos, and H.M. Moutsopoulos Impaired Urinary Excretion of Soluble IL-2 Receptors in Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis Lupus, February 1, 1992; 1(2): 105 - 109. [Abstract] [PDF]
|
|
|
|
 |
|
 L. A. Rubin and D. L. Nelson The Soluble Interleukin-2 Receptor: Biology, Function, and Clinical Application Ann Intern Med, October 15, 1990; 113(8): 619 - 627. [Abstract] [PDF]
|
|
|
|
 |
|
 M. H. Rapaport, C. G. McAllister, D. Pickar, D. L. Nelson, and S. M. Paul Elevated Levels of Soluble Interleukin 2 Receptors in Schizophrenia Arch Gen Psychiatry, March 1, 1989; 46(3): 291 - 292. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
