The Journal of Immunology, Vol 141, Issue 8 2577-2580, Copyright © 1988 by American Association of Immunologists

ARTICLES

Receptor-like nature of class I HLA: endocytosis via coated pits

JD Dasgupta, S Watkins, H Slayter and EJ Yunis
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA.

The present investigations show that class I HLA are internalized by macrophage/monocyte type cells. Anti-class I antibody-binding assays show that about 30% of class I Ag present on cell surface are endocytosed within 1 h. Electronmicroscopic investigations reveal that, like other well established receptor molecules, internalization of HLA is mediated by coated pits and coated vesicles. The endocytosed Ag are transferred from endosomes to trans-Golgi reticulum and trans-Golgi cisternae, suggesting recycling of these Ag back to the cell surface. In the presence of phorbol ester tetradecanoyl phorbol acetate, there is a modest increase in the rate of internalization. These results are consistent with the hypothesis that class I Ag on monocytes/macrophages behave like receptor molecules. Malignant transformation of monocytic cells apparently causes the loss of this property of class I Ag.

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