| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 141, Issue 5 1576-1581, Copyright © 1988 by American Association of Immunologists
ARTICLES |
JH Schumacher, A O'Garra, B Shrader, A van Kimmenade, MW Bond, TR Mosmann and RL Coffman
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304- 1140.
Four rat mAb directed against mouse IL-5 have been characterized by their ability to remove and neutralize mouse IL-5 activity in various bioassays. All four mAb absorbed IL-5 activity from solution. Although all were able to neutralize mouse IL-5 bioactivity, two were significantly more effective. These two were also able to neutralize the activity of mouse IL-5 delivered to B cells during a cognate-linked interaction with a Th cell clone. A two-site sandwich ELISA specific for mouse IL-5 was developed by using pairs of mAb. The mouse IL-5 ELISA is capable of detecting natural or mouse rIL-5 in supernatants, crude bacterial lysates, and high concentrations of mouse serum, and has a detection limit of less than 20 pg. Two of these antibodies cross- reacted with and neutralized human rIL-5, and one of these was used for development of an ELISA for human IL-5.
This article has been cited by other articles:
|
|
 |
|
  Y.-T. Chen and J. T. Kung CD1d-Independent Developmental Acquisition of Prompt IL-4 Gene Inducibility in Thymus CD161(NK1)-CD44lowCD4+CD8- T Cells Is Associated with Complementarity Determining Region 3-Diverse and Biased V{beta}2/V{beta}7/V{beta}8/V{alpha}3.2 T Cell Receptor Usage J. Immunol., November 15, 2005; 175(10): 6537 - 6550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. La Flamme, A. S. MacDonald, C. R. Huxtable, M. Carroll, and E. J. Pearce Lack of C3 Affects Th2 Response Development and the Sequelae of Chemotherapy in Schistosomiasis J. Immunol., January 1, 2003; 170(1): 470 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kanehiro, M. Lahn, M. J. Makela, A. Dakhama, A. Joetham, Y.-H. Rha, W. Born, and E. W. Gelfand Requirement for the p75 TNF-{alpha} Receptor 2 in the Regulation of Airway Hyperresponsiveness by {gamma}{delta} T Cells J. Immunol., October 15, 2002; 169(8): 4190 - 4197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Mayr, R. I. Zuberi, M. Zhang, J. de Sousa-Hitzler, K. Ngo, Y. Kuwabara, L. Yu, W.-P. Fung-Leung, and F.-T. Liu IgE-Dependent Mast Cell Activation Potentiates Airway Responses in Murine Asthma Models J. Immunol., August 15, 2002; 169(4): 2061 - 2068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dakhama, A. Kanehiro, M. J. Makela, J. E. Loader, G. L. Larsen, and E. W. Gelfand Regulation of Airway Hyperresponsiveness by Calcitonin Gene-related Peptide in Allergen Sensitized and Challenged Mice Am. J. Respir. Crit. Care Med., April 15, 2002; 165(8): 1137 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. KANEHIRO, M. LAHN, M. J. MAKELA, A. DAKHAMA, M. FUJITA, A. JOETHAM, R. J. MASON, W. BORN, and E. W. GELFAND Tumor Necrosis Factor-alpha Negatively Regulates Airway Hyperresponsiveness through gamma delta T Cells Am. J. Respir. Crit. Care Med., December 15, 2001; 164(12): 2229 - 2238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. La Flamme, E. A. Patton, and E. J. Pearce Role of Gamma Interferon in the Pathogenesis of Severe Schistosomiasis in Interleukin-4-Deficient Mice Infect. Immun., December 1, 2001; 69(12): 7445 - 7452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nakata, O. Kaminuma, K. Ogawa, H. Fujimura, K. Fushimi, H. Kikkawa, K. Naito, K. Ikezawa, R. W. Egan, and A. Mori Correlation between eosinophilia induced by CD4+ T cells and bronchial hyper-responsiveness Int. Immunol., March 1, 2001; 13(3): 329 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Erickson, T. M. Foy, and T. J. Waldschmidt Murine B1 B Cells Require IL-5 for Optimal T Cell-Dependent Activation J. Immunol., February 1, 2001; 166(3): 1531 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kobayashi, T. Miura, T. Haba, M. Sato, I. Serizawa, H. Nagai, and K. Ishizaka An Essential Role of Mast Cells in the Development of Airway Hyperresponsiveness in a Murine Asthma Model J. Immunol., April 1, 2000; 164(7): 3855 - 3861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-H. Mu, A. D. Sawitzke, and B. C. Cole Modulation of Cytokine Profiles by the Mycoplasma Superantigen Mycoplasma arthritidis Mitogen Parallels Susceptibility to Arthritis Induced by M. arthritidis Infect. Immun., March 1, 2000; 68(3): 1142 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Schwarze, M. Makela, G. Cieslewicz, A. Dakhama, M. Lahn, T. Ikemura, A. Joetham, and E. W. Gelfand Transfer of the Enhancing Effect of Respiratory Syncytial Virus Infection on Subsequent Allergic Airway Sensitization by T Lymphocytes J. Immunol., November 15, 1999; 163(10): 5729 - 5734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. D. Finkelman and S. C. Morris Development of an assay to measure in vivo cytokine production in the mouse Int. Immunol., November 1, 1999; 11(11): 1811 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Le Moine, M. Surquin, F. X. Demoor, J. C. Noel, M.-A. Nahori, M. Pretolani, V. Flamand, M. Y. Braun, M. Goldman, and D. Abramowicz IL-5 Mediates Eosinophilic Rejection of MHC Class II-Disparate Skin Allografts in Mice J. Immunol., October 1, 1999; 163(7): 3778 - 3784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. La Flamme and E. J. Pearce The Absence of IL-6 Does Not Affect Th2 Cell Development In Vivo, But Does Lead to Impaired Proliferation, IL-2 Receptor Expression, and B Cell Responses J. Immunol., May 15, 1999; 162(10): 5829 - 5837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Schwarze, G. Cieslewicz, A. Joetham, T. Ikemura, E. Hamelmann, and E. W. Gelfand CD8 T Cells Are Essential in the Development of Respiratory Syncytial Virus-Induced Lung Eosinophilia and Airway Hyperresponsiveness J. Immunol., April 1, 1999; 162(7): 4207 - 4211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Schwarze, G. Cieslewicz, E. Hamelmann, A. Joetham, L. D. Shultz, M. C. Lamers, and E. W. Gelfand IL-5 and Eosinophils Are Essential for the Development of Airway Hyperresponsiveness Following Acute Respiratory Syncytial Virus Infection J. Immunol., March 1, 1999; 162(5): 2997 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Garlisi, T. T. Kung, P. Wang, M. Minnicozzi, S. P. Umland, R. W. Chapman, D. Stelts, Y. Crawley, A. Falcone, J. G. Myers, et al. Effects of Chronic Anti-Interleukin-5 Monoclonal Antibody Treatment in a Murine Model of Pulmonary Inflammation Am. J. Respir. Cell Mol. Biol., February 1, 1999; 20(2): 248 - 255. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Girgis, M. M. Davis, and B. F. de St. Groth The Avidity Spectrum of T Cell Receptor Interactions Accounts for T Cell Anergy in a Double Transgenic Model J. Exp. Med., January 18, 1999; 189(2): 265 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xiong, G. Karupiah, S. P. Hogan, P. S. Foster, and A. J. Ramsay Inhibition of Allergic Airway Inflammation in Mice Lacking Nitric Oxide Synthase 2 J. Immunol., January 1, 1999; 162(1): 445 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Assenmacher, M. Lohning, A. Scheffold, A. Richter, S. Miltenyi, J. Schmitz, and A. Radbruch Commitment of Individual Th1-Like Lymphocytes to Expression of IFN-{gamma} Versus IL-4 and IL-10: Selective Induction of IL-10 by Sequential Stimulation of Naive Th Cells with IL-12 and IL-4 J. Immunol., September 15, 1998; 161(6): 2825 - 2832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. KRUG, T. JUNG, U. NAPP, K. WAGNER, G. SCHULTZE-WERNINGHAUS, C. HEUSSER, C. H. L. RIEGER, U. SCHAUER, and H. FABEL Frequencies of T Cells Expressing Interleukin-4 and Interleukin-5 in Atopic Asthmatic Children . Comparison with Atopic Asthmatic Adults Am. J. Respir. Crit. Care Med., September 1, 1998; 158(3): 754 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. SCHWARZE, G. CIESLEWICZ, A. JOETHAM, L. K. SUN, W. N. SUN, T. W. CHANG, E. HAMELMANN, and E. W. GELFAND Antigen-specific Immunoglobulin-A Prevents Increased Airway Responsiveness and Lung Eosinophilia after Airway Challenge in Sensitized Mice Am. J. Respir. Crit. Care Med., August 1, 1998; 158(2): 519 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Garlisi, A. Falcone, J. A. Hey, T. M. Paster, X. Fernandez, C. A. Rizzo, M. Minnicozzi, H. Jones, M. Motasim Billah, R. W. Egan, et al. Airway Eosinophils, T Cells, Th2-Type Cytokine mRNA, and Hyperreactivity in Response to Aerosol Challenge of Allergic Mice with Previously Established Pulmonary Inflammation Am. J. Respir. Cell Mol. Biol., November 1, 1997; 17(5): 642 - 651. [Abstract] [Full Text]
|
|
|
|
|
|
M. McKinnon, K. Page, I. J. Uings, M. Banks, D. Fattah, A. E.I. Proudfoot, P. Graber, C. Arod, R. Fish, T. N.C. Wells, et al. An Interleukin 5 Mutant Distinguishes between Two Functional Responses in Human Eosinophils J. Exp. Med., July 7, 1997; 186(1): 121 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Scharton-Kersten, G. Yap, J. Magram, and A. Sher Inducible Nitric Oxide Is Essential for Host Control of Persistent but Not Acute Infection with the Intracellular Pathogen Toxoplasma gondii J. Exp. Med., April 7, 1997; 185(7): 1261 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Coffman, B. Seymour, S Hudak, J Jackson, and D Rennick Antibody to interleukin-5 inhibits helminth-induced eosinophilia in mice Science, July 21, 1989; 245(4915): 308 - 310. [Abstract] [PDF]
|
|
|
|
 |
|
 B. P. England, P. Balasubramanian, I. Uings, S. Bethell, M.-J. Chen, P. J. Schatz, Q. Yin, Y.-F. Chen, E. A. Whitehorn, A. Tsavaler, et al. A potent dimeric peptide antagonist of interleukin-5 that binds two interleukin-5 receptor alpha chains PNAS, June 6, 2000; 97(12): 6862 - 6867. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
