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The Journal of Immunology, Vol 141, Issue 5 1451-1455, Copyright © 1988 by American Association of Immunologists

ARTICLES

T cell activation by purified, soluble, class I MHC molecules. Requirement for polyvalency

J McCluskey, LF Boyd, PF Highet, J Inman and DH Margulies
Molecular Biology Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

To examine the nature of the interaction of the TCR with the MHC class I Ag, we have studied the stimulation requirements of an H-2Dd-reactive T cell hybridoma, using a homogeneous, purified preparation of a molecularly engineered soluble counterpart of the class I Ag, H- 2Dd/Q10b. We demonstrate that this monovalent, soluble MHC Ag is incapable of stimulating the release of IL-2 from this T cell hybridoma. However, the same preparation of the purified protein can elicit a dose-dependent response when made multivalent either by covalent coupling to soluble, high m.w. dextran or to agarose beads, or by adsorption to polystyrene tissue culture plates.


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