The Journal of Immunology, Vol 141, Issue 4 1231-1235, Copyright © 1988 by American Association of Immunologists

ARTICLES

IL-2 production in human T lymphotropic virus I-infected leukemic T lymphocytes analyzed by in situ hybridization

N Goebels, I Waase, K Pfizenmaier and M Kronke
Klinische Arbeitsgruppe, Max-Planck-Gesellschaft, Gottingen, Federal Republic of Germany.

In situ hybridization studies were performed with 35S-labeled anti- sense RNA probes to study IL-2 mRNA expression in three human T lymphotropic virus I-infected T cell lines at the single cell level. In HuT 102, MT-2, and MT-4 cells, IL-2 mRNA-expressing cells were identified, occurring at frequencies of 2 x 10(-2), 8 x 10(-3), and 5 x 10(-3), respectively. In these cell lines, IL-2 mRNA was not detectable in RNA extracted from whole adult T cell leukemia cell populations because of dilution by other RNA species from the vast majority of cells that do not contain IL-2 mRNA. The data indicate the possibility of paracrine growth stimulation via IL-2 and its receptor even in those human T lymphotropic virus I-infected T cell populations that apparently lack IL-2 activity when analyzed by conventional assay procedures.

This article has been cited by other articles:

				P. Hollsberg Mechanisms of T-Cell Activation by Human T-Cell Lymphotropic Virus Type I Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 308 - 333. [Abstract] [Full Text] [PDF]

				P. Hollsberg and D. A. Hafler Pathogenesis of Diseases Induced by Human Lymphotropic Virus Type I Infection N. Engl. J. Med., April 22, 1993; 328(16): 1173 - 1182. [Full Text]