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The Journal of Immunology, Vol 141, Issue 4 1174-1179, Copyright © 1988 by American Association of Immunologists

ARTICLES

Evidence for two discrete phases of IL-2 production in bovine lymphocytes

AD Weinberg, NS Magnuson, R Reeves, CR Wyatt and JA Magnuson
Department of Microbiology, Washington State University, Pullman 99164.

Regulation of IL-2 production in Con A-stimulated bovine lymph node cells was studied by following the time course of IL-2 synthesis and secretion over a 24-h period. A biphasic time course curve of IL-2 secretion was observed after stimulation with Con A alone or with Con A plus PMA. Both phases of IL-2 production were confirmed by three separate biochemical techniques: IL-2 protein bioassay, mRNA Northern blots, and anti-IL-2 antibody detection. Bovine IL-2 transcripts were detected as early as 2 h after Con A stimulation. The early phase of IL- 2 protein secretion was initially detected 3 h after Con A stimulation, and the late phase occurred between 10 and 16 h. Both phases of IL-2 production were enhanced when PMA was added to the Con A stimulation. Each phase of IL-2 protein secretion was preceded by the accumulation of IL-2 mRNA.


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[Abstract] [Full Text] [PDF]


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