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The Journal of Immunology, Vol 141, Issue 2 355-362, Copyright © 1988 by American Association of Immunologists
ARTICLES |
R Ceredig
Department of Experimental Pathology, John Curtin School of Medical Research, Canberra City, Australia.
A two-color flow cytometric analysis was carried out on 14-day fetal mouse thymus lobes maintained for various lengths of time in organ culture. Kinetic analysis showed that the proportions of CD4- and CD8- defined subpopulations changed with time. In particular, there appeared to be accumulation of J11d-, CD4/CD8 double-negative and J11d-, Ly-6A+ single-positive cells, a phenotype associated with "mature" cells. Gated two-color analysis of CD4/CD8 single-positive cells showed that they contained distinct subpopulations as defined by mAb to CD3, TCR (F23.1), Ly-6A, Ly-6C, LFA-1, and J11d. Thus, the precursor cells found in the 14-day fetal mouse thymus were capable of generating all the subpopulations of CD4/CD8-defined single-positive and double-negative cells seen in the normal adult mouse thymus.
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