The Journal of Immunology, Vol 141, Issue 12 4089-4093, Copyright © 1988 by American Association of Immunologists

ARTICLES

Anti-Ig antibody inhibits the phorbol ester-induced stimulation of peritoneal B cells

TL Rothstein and DL Kolber
Evans Memorial Department of Clinical Research, Boston University Medical Center, MA 02118.

Peritoneal B cells are stimulated to enter S phase by phorbol esters acting alone, in the absence of a co-mitogen. Anti-Ig antibody inhibited the stimulation of peritoneal B cells induced by phorbol esters and this inhibition was apparent throughout the time course of PMA-induced stimulation. Assessment of inhibition after depletion of Ly- 1+ B cells suggested that PMA-induced stimulation of both Ly-1+ and Ly- 1- B cells was affected by anti-Ig. Inhibition produced by anti-Ig was substantially reduced after even a brief delay in the addition of antibody to PMA-stimulated B cells. Anti-Ig antibody may counteract early events triggered by PMA within minutes of peritoneal B cell stimulation.

This article has been cited by other articles:

				J. M. Mataraza, J. R. Tumang, M. R. Gumina, S. M. Gurdak, T. L. Rothstein, and T. C. Chiles Disruption of Cyclin D3 Blocks Proliferation of Normal B-1a Cells, but Loss of Cyclin D3 Is Compensated by Cyclin D2 in Cyclin D3-Deficient Mice J. Immunol., July 15, 2006; 177(2): 787 - 795. [Abstract] [Full Text] [PDF]

				R. Frances, J. R. Tumang, and T. L. Rothstein Cutting Edge: B-1 Cells Are Deficient in Lck: Defective B Cell Receptor Signal Transduction in B-1 Cells Occurs in the Absence of Elevated Lck Expression J. Immunol., July 1, 2005; 175(1): 27 - 31. [Abstract] [Full Text] [PDF]

				S.-C. Wong, W.-K. Chew, J. E.-L. Tan, A. J. Melendez, F. Francis, and K.-P. Lam Peritoneal CD5+ B-1 Cells Have Signaling Properties Similar to Tolerant B Cells J. Biol. Chem., August 16, 2002; 277(34): 30707 - 30715. [Abstract] [Full Text] [PDF]

				T. L. Rothstein Two B-1 or Not To Be One J. Immunol., May 1, 2002; 168(9): 4257 - 4261. [Abstract] [Full Text] [PDF]

				D. A. Tanguay, T. P. Colarusso, C. Doughty, S. Pavlovic-Ewers, T. L. Rothstein, and T. C. Chiles Cutting Edge: Differential Signaling Requirements for Activation of Assembled Cyclin D3-cdk4 Complexes in B-1 and B-2 Lymphocyte Subsets J. Immunol., April 1, 2001; 166(7): 4273 - 4277. [Abstract] [Full Text] [PDF]

				G. J. Silverman, S. P. Cary, D. C. Dwyer, L. Luo, R. Wagenknecht, and V. E. Curtiss A B Cell Superantigen-induced Persistent "Hole" in the B-1 Repertoire J. Exp. Med., July 3, 2000; 192(1): 87 - 98. [Abstract] [Full Text] [PDF]

				D. A. Tanguay, T. P. Colarusso, S. Pavlovic, M. Irigoyen, R. G. Howard, J. Bartek, T. C. Chiles, and T. L. Rothstein Early Induction of Cyclin D2 Expression in Phorbol Ester-responsive B-1 Lymphocytes J. Exp. Med., June 7, 1999; 189(11): 1685 - 1690. [Abstract] [Full Text] [PDF]

				N. Baumgarth, O. C. Herman, G. C. Jager, L. Brown, L. A. Herzenberg, and L. A. Herzenberg Innate and acquired humoral immunities to influenza virus are mediated by distinct arms of the immune system PNAS, March 2, 1999; 96(5): 2250 - 2255. [Abstract] [Full Text] [PDF]

				D. A. Cockayne, T. Muchamuel, J. C. Grimaldi, H. Muller-Steffner, T. D. Randall, F. E. Lund, R. Murray, F. Schuber, and M. C. Howard Mice Deficient for the Ecto-Nicotinamide Adenine Dinucleotide Glycohydrolase CD38 Exhibit Altered Humoral Immune Responses Blood, August 15, 1998; 92(4): 1324 - 1333. [Abstract] [Full Text] [PDF]

				J. G. Karras, Z. Wang, L. Huo, R. G. Howard, D. A. Frank, and T. L. Rothstein Signal Transducer and Activator of Transcription-3 (STAT3) Is Constitutively Activated in Normal, Self-renewing B-1 Cells but Only Inducibly Expressed in Conventional B Lymphocytes J. Exp. Med., March 17, 1997; 185(6): 1035 - 1042. [Abstract] [Full Text] [PDF]

				G. Bikah, J. Carey, J. R. Ciallella, A. Tarakhovsky, and S. Bondada CD5-Mediated Negative Regulation of Antigen Receptor-Induced Growth Signals in B-1 B Cells Science, December 13, 1996; 274(5294): 1906 - 1909. [Abstract] [Full Text]