The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spits, H.
Right arrow Articles by de Vries, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spits, H.
Right arrow Articles by de Vries, J. E.

The Journal of Immunology, Vol 141, Issue 1 29-36, Copyright © 1988 by American Association of Immunologists

ARTICLES

IL-4 inhibits IL-2-mediated induction of human lymphokine-activated killer cells, but not the generation of antigen-specific cytotoxic T lymphocytes in mixed leukocyte cultures

H Spits, H Yssel, X Paliard, R Kastelein, C Figdor and JE de Vries
UNICET, Laboratory for Immunological Research, Dardilly, France.

Human rIL-4 was studied for its capacity to induce lymphokine-activated killer (LAK) cell activity. In contrast to IL-2, IL-4 was not able to induce LAK cell activity in cell cultures derived from peripheral blood. IL-4 added simultaneously with IL-2 to such cultures suppressed IL-2-induced LAK cell activity measured against Daudi and the melanoma cell line MEWO in a dose-dependent way. IL-4 also inhibited the induction of LAK cell activity in CD2+, CD3-, CD4-, CD8- cells, suggesting that IL-4 acts directly on LAK precursor cells. IL-4 added 24 h after the addition of IL-2 failed to inhibit the generation of LAK cell activity. Cytotoxic activity of various types of NK cell clones was not affected after incubation in IL-4 for 3 days, indicating that IL-4 does not affect the activity of already committed killer cells. No significant differences were observed in the percentages of Tac+, NKH- 1+ and CD16+ cells after culturing PBL in IL-2, IL-4 or combinations of IL-2 and IL-4 for 3 days. IL-4 also inhibited the activation of non- specific cytotoxic activity in MLC, as measured against K-562 and MEWO cells. In contrast, the Ag-specific CTL activity against the stimulator cells was augmented by IL-4. Collectively, these data indicate that IL- 4 prevents the activation of LAK cell precursors by IL-2, but does not inhibit the generation of Ag-specific CTL.


This article has been cited by other articles:


Home page
 BloodHome page
A. Catalano, P. Caprari, S. Moretti, M. Faronato, L. Tamagnone, and A. Procopio
Semaphorin-3A is expressed by tumor cells and alters T-cell signal transduction and function
Blood, April 15, 2006; 107(8): 3321 - 3329.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Seo, Y. Tokura, F. Furukawa, and M. Takigawa
Down-Regulation of Tumoricidal NK and NK T Cell Activities by MHC Kb Molecules Expressed on Th2-Type {gamma}{delta} T and {alpha}{beta} T Cells Coinfiltrating in Early B16 Melanoma Lesions
J. Immunol., October 15, 1998; 161(8): 4138 - 4145.
[Abstract] [Full Text] [PDF]

Home page
 Arch SurgHome page
H. Stotter and M. T. Lotze
Human Lymphokine-Activated Killer Cell Activity: Role of IL-2, IL-4, and IL-7
Arch Surg, December 1, 1991; 126(12): 1525 - 1530.
[Abstract] [PDF]

Home page
 ScienceHome page
D. Eardley and M. Koshland
Glycosylphosphatidylinositol: a candidate system for interleukin-2 signal transduction
Science, January 4, 1991; 251(4989): 78 - 81.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1988 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1988 by The American Association of Immunologists, Inc. All rights reserved.