| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 140, Issue 9 3006-3013, Copyright © 1988 by American Association of Immunologists
ARTICLES |
LE Bermudez and LS Young
Kuzell Institute for Arthritis and Infectious Diseases, Medical Research Institute at Pacific Presbyterian Medical Center, San Francisco, CA 94115.
Organisms belonging to the Mycobacterium avium complex (MAC) are the most common bacterial pathogens in patients with AIDS but factors associated with the activation of cellular defense mechanisms against this atypical mycobacterium have not been defined. Peritoneal macrophages harvested from a chronic MAC infection in C57 black mice are able to kill approximately 86% of intracellular MAC in contrast to 0 to 20% killing by unstimulated human and mouse macrophages in vitro. The availability of human rTNF-alpha, rIFN-gamma, and rIL-2 permitted evaluation of the role of each of these lymphokines/monokines, alone or in combination, in activating macrophages in vitro to kill MAC. Human monocyte-derived macrophages were cultured in vitro, stimulated with rIL-2, rIFN-gamma, or rTNF, and then infected with MAC (serovars 1 and 8). Mouse peritoneal macrophages were harvested, cultured in vitro, and stimulated with rIFN-gamma. rTNF (10(4) U/ml) was associated with a modest increase of intracellular killing of MAC (58 +/- 5%) even when utilized 24 or 48 h after macrophage infection or when administered for 5 consecutive days after infection (78.1 +/- 4%). Both human and murine IFN-gamma were associated with increased intracellular growth of MAC (32 +/- 4% for murine and 38 +/- 3% for human macrophages). However, intracellular killing (53 +/- 6% compared with control) was observed after 6 days of treatment with IFN-gamma. This latter effect was fully blocked by anti-TNF antibody, whereas rIL-2 alone did not augment the intracellular killing of MAC by human macrophages. rTNF plus either rIFN-gamma or rIL-2 triggered significant increases in superoxide anion production, but subsequent MAC killing was no greater than with rTNF alone. Treatment of macrophages with 10 U/ml of rTNF followed by rIL-2 (200 U/ml) was associated with 68% of intracellular killing. TNF seems to be an important monokine, promoting activation of mycobactericidal mechanisms in human macrophages.
This article has been cited by other articles:
|
|
 |
|
  O. Chacon, L. E. Bermudez, D. K. Zinniel, H. K. Chahal, R. J. Fenton, Z. Feng, K. Hanford, L. G. Adams, and R. G. Barletta Impairment of D-alanine biosynthesis in Mycobacterium smegmatis determines decreased intracellular survival in human macrophages Microbiology, May 1, 2009; 155(5): 1440 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A S-Y Leong, P Wannakrairot, and T Y-M Leong Apoptosis is a major cause of so-called "caseous necrosis" in mycobacterial granulomas in HIV-infected patients J. Clin. Pathol., March 1, 2008; 61(3): 366 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Florido and R. Appelberg Characterization of the Deregulated Immune Activation Occurring at Late Stages of Mycobacterial Infection in TNF-Deficient Mice J. Immunol., December 1, 2007; 179(11): 7702 - 7708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Vazquez, T. Greenwell-Wild, S. Rekka, J. M. Orenstein, and S. M. Wahl Mycobacterium avium-induced SOCS contributes to resistance to IFN-{gamma}-mediated mycobactericidal activity in human macrophages J. Leukoc. Biol., November 1, 2006; 80(5): 1136 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-B. Lee and J. S. Schorey Activation and Mitogen-Activated Protein Kinase Regulation of Transcription Factors Ets and NF-{kappa}B in Mycobacterium-Infected Macrophages and Role of These Factors in Tumor Necrosis Factor Alpha and Nitric Oxide Synthase 2 Promoter Function Infect. Immun., October 1, 2005; 73(10): 6499 - 6507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Lopez, E. Clark, and V. L. Shepherd Surfactant protein A enhances Mycobacterium avium ingestion but not killing by rat macrophages J. Leukoc. Biol., October 1, 2003; 74(4): 523 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Johnson, E. Ssekasanvu, A. Okwera, H. Mayanja, C. S. Hirsch, J. G. Nakibali, D. D. Jankus, K. D. Eisenach, W. H. Boom, J. J. Ellner, et al. Randomized Trial of Adjunctive Interleukin-2 in Adults with Pulmonary Tuberculosis Am. J. Respir. Crit. Care Med., July 15, 2003; 168(2): 185 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Barthel, A. V. Tsytsykova, A. K. Barczak, E. Y. Tsai, C. C. Dascher, M. B. Brenner, and A. E. Goldfeld Regulation of Tumor Necrosis Factor Alpha Gene Expression by Mycobacteria Involves the Assembly of a Unique Enhanceosome Dependent on the Coactivator Proteins CBP/p300 Mol. Cell. Biol., January 15, 2003; 23(2): 526 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wagner, F. J. Sangari, S. Kim, M. Petrofsky, and L. E. Bermudez Mycobacterium avium infection of macrophages results in progressive suppression of interleukin-12 production in vitro and in vivo J. Leukoc. Biol., January 1, 2002; 71(1): 80 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Fratazzi, N. Manjunath, R. D. Arbeit, C. Carini, T. A. Gerken, B. Ardman, E. Remold-O'Donnell, and H. G. Remold A Macrophage Invasion Mechanism for Mycobacteria Implicating the Extracellular Domain of Cd43 J. Exp. Med., July 17, 2000; 192(2): 183 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Miltner and L. E. Bermudez Mycobacterium avium Grown in Acanthamoeba castellanii Is Protected from the Effects of Antimicrobials Antimicrob. Agents Chemother., July 1, 2000; 44(7): 1990 - 1994. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. P. Mizgerd, J. J. Peschon, and C. M. Doerschuk Roles of Tumor Necrosis Factor Receptor Signaling during Murine Escherichia coli Pneumonia Am. J. Respir. Cell Mol. Biol., January 1, 2000; 22(1): 85 - 91. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. STOLTZ, S. NELSON, J. K. KOLLS, P. ZHANG, R. P. BOHM Jr., M. MURPHEY-CORB, and G. J. BAGBY In Vitro Ethanol Suppresses Alveolar Macrophage TNF-alpha during Simian Immunodeficiency Virus Infection Am. J. Respir. Crit. Care Med., January 1, 2000; 161(1): 135 - 140. [Abstract] [Full Text]
|
|
|
|
|
|
H.-P. KUO, C.-H. WANG, K.-S. HUANG, H.-C. LIN, C.-T. YU, C.-Y. LIU, and L.-C. LU Nitric Oxide Modulates Interleukin-1beta and Tumor Necrosis Factor-alpha Synthesis by Alveolar Macrophages in Pulmonary Tuberculosis Am. J. Respir. Crit. Care Med., January 1, 2000; 161(1): 192 - 199. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Owaki, A. Meneshian, K. Maemura, S. Takao, D. Wang, K. C. Fuh, G. B. Bulkley, and A. S. Klein Endothelial cells potentiate phagocytic killing by macrophages via platelet-activating factor release Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H269 - H276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Pahlevan, D. J. M. Wright, C. Andrews, K. M. George, P. L. C. Small, and B. M. Foxwell The Inhibitory Action of Mycobacterium ulcerans Soluble Factor on Monocyte/T Cell Cytokine Production and NF-{kappa}B Function J. Immunol., October 1, 1999; 163(7): 3928 - 3935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Juffermans, A. Verbon, S. J. H. van Deventer, H. van Deutekom, J. T. Belisle, M. E. Ellis, P. Speelman, and T. van der Poll Elevated Chemokine Concentrations in Sera of Human Immunodeficiency Virus (HIV)-Seropositive and HIV-Seronegative Patients with Tuberculosis: a Possible Role for Mycobacterial Lipoarabinomannan Infect. Immun., August 1, 1999; 67(8): 4295 - 4297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. BONAY, F. BOUCHONNET, V. PELICIC, B. LAGIER, M. GRANDSAIGNE, D. LECOSSIER, A. GRODET, M. VOKURKA, B. GICQUEL, and A. J. HANCE Effect of Stimulation of Human Macrophages on Intracellular Survival of Mycobacterium bovis Bacillus Calmette-Guerin . Evaluation with a Mycobacterial Reporter Strain Am. J. Respir. Crit. Care Med., May 1, 1999; 159(5): 1629 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-M. TCHOU-WONG, O. TANABE, C. CHI, T.-A. YIE, and W. N. ROM Activation of NF-kappa B in Mycobacterium tuberculosis- induced Interleukin-2 Receptor Expression in Mononuclear Phagocytes Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): 1323 - 1329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sano, T. Shimizu, K. Sato, H. Kawauchi, S. Kawahara, and H. Tomioka Therapeutic Effects of Benzoxazinorifamycin KRM-1648 Administered Alone or in Combination with a Half-Sized Secretory Leukocyte Protease Inhibitor or the Nonsteroidal Anti-Inflammatory Drug Diclofenac Sodium against Mycobacterium avium Complex Infection in Mice Antimicrob. Agents Chemother., February 1, 1999; 43(2): 360 - 364. [Abstract] [Full Text]
|
|
|
|
|
|
T. R. Garbe, N. S. Hibler, and V. Deretic Response to Reactive Nitrogen Intermediates in Mycobacterium tuberculosis: Induction of the 16-Kilodalton alpha -Crystallin Homolog by Exposure to Nitric Oxide Donors Infect. Immun., January 1, 1999; 67(1): 460 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bala, K. L. Hastings, K. Kazempour, S. Inglis, and W. L. Dempsey Inhibition of Tumor Necrosis Factor Alpha Alters Resistance to Mycobacterium avium Complex Infection in Mice Antimicrob. Agents Chemother., September 1, 1998; 42(9): 2336 - 2341. [Abstract] [Full Text]
|
|
|
|
|
|
T. YONEDA and J. J. ELLNER CD4+ T Cell and Natural Killer Cell-dependent Killing of Mycobacterium tuberculosis by Human Monocytes Am. J. Respir. Crit. Care Med., August 1, 1998; 158(2): 395 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Everest, M. Roberts, and G. Dougan Susceptibility to Salmonella typhimurium Infection and Effectiveness of Vaccination in Mice Deficient in the Tumor Necrosis Factor Alpha p55 Receptor Infect. Immun., July 1, 1998; 66(7): 3355 - 3364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Holsti, J. S. Schorey, E. J. Brown, and P. M. Allen Identification of Epitopes of Fibronectin Attachment Protein (FAP-A) of Mycobacterium avium Which Stimulate Strong T-Cell Responses in Mice Infect. Immun., March 1, 1998; 66(3): 1261 - 1264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Via, R. Fratti, M McFalone, E Pagan-Ramos, D Deretic, and V Deretic Effects of cytokines on mycobacterial phagosome maturation J. Cell Sci., January 4, 1998; 111(7): 897 - 905. [Abstract] [PDF]
|
|
|
|
|
|
H. Yu, M. Hanes, C. E. Chrisp, J. C. Boucher, and V. Deretic Microbial Pathogenesis in Cystic Fibrosis: Pulmonary Clearance of Mucoid Pseudomonas aeruginosa and Inflammation in a Mouse Model of Repeated Respiratory Challenge Infect. Immun., January 1, 1998; 66(1): 280 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Page, E. M. Roberts, J. J. Peschon, and S. M. Hedrick TNF Receptor-Deficient Mice Reveal Striking Differences Between Several Models of Thymocyte Negative Selection J. Immunol., January 1, 1998; 160(1): 120 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Crowle Interferon-Gamma and Mycobacterial Infections Ann Intern Med, October 1, 1988; 109(7): 595 - 596. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
