The Journal of Immunology, Vol 140, Issue 4 1148-1152, Copyright © 1988 by American Association of Immunologists

ARTICLES

Lack of an obligate role for IFN-gamma in the primary in vitro mixed lymphocyte response

RP Bucy, DW Hanto, E Berens and RD Schreiber
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.

We have investigated the relative importance of two lymphokines, IL-2 and IFN-gamma, in the primary murine MLR. Three separate lines of evidence indicate that IL-2 and not IFN-gamma is the relevant lymphokine for both proliferation and activation of CTL in these cultures. No obligate role for IFN-gamma was found. First, CTL activation in the presence of high dose cyclosporin A was partially reconstituted with IL-2, although no detectable IFN-gamma was produced in such cultures. In addition, IFN-gamma could not reconstitute cyclosporin-inhibited cultures. Second, inclusion of a high concentration of several distinct anti-IFN-gamma antibodies failed to inhibit MLR cultures. Third, CTL activation by stimulator cells inactivated by UV irradiation was reconstituted by IL-2, but not by IFN- gamma. These data do not support an autocrine role of IFN-gamma in CTL activation and confirm the importance of IL-2 in the primary murine MLR.

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