The Journal of Immunology, Vol 140, Issue 11 3736-3744, Copyright © 1988 by American Association of Immunologists

ARTICLES

T cell-dependent activation of B cell proliferation and differentiation by immobilized monoclonal antibodies to CD3

S Hirohata, DF Jelinek and PE Lipsky
Department of Internal Medicine, University of Texas Health Science Center, Dallas 75235.

The capacity of mAb directed at the CD3 molecular complex (64.1) to induce T cell-dependent B cell proliferation and differentiation was examined. Coculture of B cells with mitomycin C-treated T4 cells (T4 mito) stimulated by immobilized 64.1 resulted in marked B cell proliferation and Ig-secreting cells (ISC) generation in the absence of any additional stimulation. The magnitude of the B cell responses induced by immobilized 64.1-stimulated T4 mito was far greater than that induced by other stimuli, such as Staphylococcus aureus plus factors produced by mitogen-activated T cells, PWM, or soluble 64.1. The induction of maximal B cell responsiveness required direct contact between activated T cells and responding B cells. Of note, immobilized 64.1 also induced B cell proliferation and ISC generation in the presence of mitomycin C-treated T8 cells. By contrast, immobilized 64.1 stimulated T4 or T8 cells that had not been treated with mitomycin C induced very modest ISC generation and suppressed B cell responses supported by T4 mito even in the presence of exogenous IL-2 or factors produced by mitogen-activated T cells. The interactions between T and B cells in these cultures not only induced B cell responses, but also enhanced the production of IL-2 by activated T cells. Increased IL-2 production was facilitated when culture conditions afforded the opportunity for contact between B cells and activated T cells. These results indicate that the establishment of interactions between B cells and anti-CD3-stimulated T4 or T8 cells provides all of the signals necessary for proliferation and differentiation of B cells without other stimuli and also augments the production of lymphokines by the activated T cells. The data emphasize the role of Ag-nonspecific interactions between B cells and T cells in promoting polyclonal responses of both cell types.

This article has been cited by other articles:

				N. H. R. Litjens, M. Huisman, D. Hijdra, B. M. N. Lambrecht, K. J. Stittelaar, and M. G. H. Betjes IL-2 Producing Memory CD4+ T Lymphocytes Are Closely Associated with the Generation of IgG-Secreting Plasma Cells J. Immunol., September 1, 2008; 181(5): 3665 - 3673. [Abstract] [Full Text] [PDF]

				S. Kuchen, R. Robbins, G. P. Sims, C. Sheng, T. M. Phillips, P. E. Lipsky, and R. Ettinger Essential Role of IL-21 in B Cell Activation, Expansion, and Plasma Cell Generation during CD4+ T Cell-B Cell Collaboration J. Immunol., November 1, 2007; 179(9): 5886 - 5896. [Abstract] [Full Text] [PDF]

				S. Hirohata, T. Yanagida, T. Tomita, H. Yoshikawa, and T. Ochi Bone marrow CD34+ progenitor cells stimulated with stem cell factor and GM-CSF have the capacity to activate IgD- B cells through direct cellular interaction J. Leukoc. Biol., June 1, 2002; 71(6): 987 - 995. [Abstract] [Full Text] [PDF]

				S Hirohata and K Nakanishi Antiribosomal P protein antibody in human systemic lupus erythematosus reacts specifically with activated T cells Lupus, September 1, 2001; 10(9): 612 - 621. [Abstract] [PDF]

				C. H. Kim, L. S. Rott, I. Clark-Lewis, D. J. Campbell, L. Wu, and E. C. Butcher Subspecialization of CXCR5+ T Cells: B Helper Activity Is Focused in a Germinal Center-localized Subset of CXCR5+ T Cells J. Exp. Med., June 18, 2001; 193(12): 1373 - 1382. [Abstract] [Full Text] [PDF]

				G. S. Ford, C. H. Yin, B. Barnhart, K. Sztam, and L. R. Covey CD40 Ligand Exerts Differential Effects on the Expression of I{gamma} Transcripts in Subclones of an IgM+ Human B Cell Lymphoma Line J. Immunol., January 15, 1998; 160(2): 595 - 605. [Abstract] [Full Text] [PDF]