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The Journal of Immunology, Vol 140, Issue 10 3508-3514, Copyright © 1988 by American Association of Immunologists

ARTICLES

Spontaneous shift of anti-DNA antibody idiotypes in systemic lupus erythematosus

R Suenaga, PA Munoz, SW Bright and NI Abdou
Department of Medicine, University of Kansas Medical Center, Kansas City 66103.

Spontaneous shift in Id expression of polyclonal anti-DNA antibodies in a patient, BS, with SLE was investigated. BS had active lupus nephritis in 1982 and developed central nervous system lupus in 1986 without evidence of active nephritis. Two rabbit polyclonal anti-Id (BS-82 and BS-86 R-anti-Id) were raised against affinity-purified anti-DNA antibodies prepared from 1982 serum (BS-82) and 1986 serum (BS-86), respectively. In addition, murine monoclonal anti-Id was prepared against BS-82 Id. Direct binding assays showed that all three anti-Id had preferential binding to the immunizing anti-DNA antibodies (the homologous Id) and poor binding to anti-DNA antibodies prepared from the different dated sample of BS. This was confirmed by inhibition assays of binding of anti-Id to the homologous Id by various Id. Moreover, inhibition assays of binding of various Id to DNA by the R- anti-Id showed that the R-anti-Id was the most effective inhibitor for the homologous Id. Testing for Id expression in serial (1982 to 1986) serum samples of BS with the R-anti-Id as probes showed that BS-82 Id declined and was undetectable after October, 1984, whereas BS-86 Id was first detectable in July, 1985, and increased by June, 1986. These results clearly demonstrate spontaneous shifts in Id expression of human anti-DNA antibodies. The phenomenon of Id shift should be considered in any future strategy for the diagnosis and therapy of human autoimmune disease by anti-Id.


This article has been cited by other articles:


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W M Williams and D A Isenberg
Naturally occurring anti-idiotypic antibodies reactive with anti-DNA antibodies in systemic lupus erythematosus
Lupus, March 1, 1998; 7(3): 164 - 175.
[Abstract] [PDF]

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R. Suenaga and N. I. Abdou
Lupus-Derived Human Monoclonal IgM anti-DNA Antibody Displays Monospecificity, High Affinity and Private Idiotype Specificity
Lupus, December 1, 1992; 1(6): 363 - 368.
[Abstract] [PDF]


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