The Journal of Immunology, Vol 140, Issue 10 3286-3289, Copyright © 1988 by American Association of Immunologists

ARTICLES

Monoclonal anti-idiotypic antibodies to an I-J interacting molecule inhibit suppression in an H-2 restricted way [published erratum appears in J Immunol 1990 Oct 15;145(8):2773]

B Jaureguiberry, L Liao, V Kuchroo, ME Dorf and B Diamond
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.

We have obtained 10 mAb from two independent fusions that are anti- idiotypic to the combining site of an anti-I-Jk antibody. These mAb block Ts cell function isn a genetically restricted manner in vitro and in vivo and recognize a determinant on macrophage membranes. In addition, they do not affect the I-Ek-restricted activation of a Th cell line specific for pigeon heart cytochrome c. We conclude that these mAb may recognize a molecule other than conventional I-Ek on cells interacting with Ts cells that is involved in mediating Ts activity. The molecule recognized may be a modified I-Ek molecule or a molecule not encoded by the genes encoding I-Ek.