| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 139, Issue 8 2579-2584, Copyright © 1987 by American Association of Immunologists
ARTICLES |
JL Rodriguez-Sanchez, C Gelpi, C Juarez and JA Hardin
Department of Immunology, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
We identified a patient (CAG) with scleroderma whose serum contained a high titer of IgG class antibodies that stained nucleoli in a pattern of independent tiny spots. When tested on isolated chromosomes, these antibodies selectively stained the nucleolus-organizing regions (NOR) of chromosomes 13, 14, 15, 21, and 22. These staining patterns were not altered when substrate cells and chromosomes were treated with RNase, 0.1 M HC1, or 4 M urea, but they were abolished by treatment with DNase and trypsin. Immunoblots performed with serum CAG on isolated nucleolar substrates identified a protein antigen of approximately 90 kDa. Antibodies affinity-purified from this protein selectively stained nucleoli and NOR chromosomal regions. Therefore, this protein is the antigen that accounts for the ability of serum CAG to recognize the NOR. In a search for the NOR 90-kDa specificity among 254 patients with various rheumatic diseases, we found nine additional patients whose sera stained metaphase chromosomes selectively at the NOR. Sera from five of them (three with scleroderma, two of unknown diagnosis) recognized a protein that electrophoretically co-migrated with the CAG antigen. Thus, scleroderma is present in at least four of six who appear to have this specificity. We conclude that autoantibodies to the NOR 90-kDa antigen have an association with scleroderma and may be useful diagnostically and as a probe for further studies of the biology of the cell nucleolus.
This article has been cited by other articles:
|
|
 |
|
  T. Sittler, J. Zhou, J. Park, N. K. Yuen, S. Sarantopoulos, J. Mollick, R. Salgia, A. Giobbie-Hurder, G. Dranoff, and F. S. Hodi Concerted Potent Humoral Immune Responses to Autoantigens Are Associated with Tumor Destruction and Favorable Clinical Outcomes without Autoimmunity Clin. Cancer Res., June 15, 2008; 14(12): 3896 - 3905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Forne, M. Carrascal, L. Martinez-Lostao, J. Abian, J. L. Rodriguez-Sanchez, and C. Juarez Identification of the Autoantigen HB as the Barrier-to-Autointegration Factor J. Biol. Chem., December 12, 2003; 278(50): 50641 - 50644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Ulanet, M. Torbenson, C. V. Dang, L. Casciola-Rosen, and A. Rosen Unique conformation of cancer autoantigen B23 in hepatoma: A mechanism for specificity in the autoimmune response PNAS, October 14, 2003; 100(21): 12361 - 12366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Casciola-Rosen, F. Wigley, and A. Rosen Scleroderma Autoantigens Are Uniquely Fragmented by Metal-catalyzed Oxidation Reactions: Implications for Pathogenesis J. Exp. Med., January 6, 1997; 185(1): 71 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Pluta and W. C. Earnshaw Specific Interaction between Human Kinetochore Protein CENP-C and a Nucleolar Transcriptional Regulator J. Biol. Chem., August 2, 1996; 271(31): 18767 - 18774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Roussel, C Andre, C Masson, G Geraud, and D Hernandez-Verdun Localization of the RNA polymerase I transcription factor hUBF during the cell cycle J. Cell Sci., January 2, 1993; 104(2): 327 - 337. [Abstract] [PDF]
|
|
|
|
|
|
R. M. Rodrigo, M. C. Rendon, J. Torreblanca, G. Garcia-Herdugo, and F. J. Moreno Characterization and immunolocalization of RNA polymerase I transcription factor UBF with anti-NOR serum in protozoa, higher plant and vertebrate cells J. Cell Sci., December 1, 1992; 103(4): 1053 - 1063. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
