The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scott, M. G.
Right arrow Articles by Nahm, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scott, M. G.
Right arrow Articles by Nahm, M. H.

The Journal of Immunology, Vol 138, Issue 10 3325-3331, Copyright © 1987 by American Association of Immunologists

ARTICLES

Human antibodies to phosphocholine. IgG anti-PC antibodies express restricted numbers of V and C regions

MG Scott, DE Briles, PG Shackelford, DS Smith and MH Nahm

We examined the IgG subclass composition and isoelectric focusing (IEF) spectrotype pattern of naturally occurring human IgG antibodies that bind phosphocholine (PC) and found direct evidence for restricted expression of both V and C regions among these antibodies. In most individuals, the isotype of these IgG anti-PC antibodies was primarily IgG2. However, serum from some individuals contained significant amounts of IgG1 and IgG3 anti-PC antibodies. We also found that in individual sera, anti-PC antibodies are pauciclonal, as demonstrated by restricted spectrotypic patterns of the anti-PC antibodies. The IEF pattern of these antibodies were for the most part unique for each individual. In some sera, certain anti-PC antibodies with isoelectric points of basic pH bound PC conjugated to bovine serum albumin (PC-BSA) but did not bind pneumococcal C-carbohydrate bearing PC determinants. In two individuals, we found that the spectrotypes that bound only PC- BSA were of the IgG1 subclass. Taken together, these findings demonstrate that within individual sera, human antibodies to PC are quite restricted in both V and C region expression, and furthermore, these V and C regions of human Ig may not randomly associate.


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
C. E. Frasch and N. F. Concepcion
Specificity of Human Antibodies Reactive with Pneumococcal C Polysaccharide
Infect. Immun., April 1, 2000; 68(4): 2333 - 2337.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H. A. Schenkein, J. C. Gunsolley, A. M. Best, M. T. Harrison, C.-L. Hahn, J. Wu, and J. G. Tew
Antiphosphorylcholine Antibody Levels Are Elevated in Humans with Periodontal Diseases
Infect. Immun., September 1, 1999; 67(9): 4814 - 4818.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
L.-a. Pirofski and A. Casadevall
Use of Licensed Vaccines for Active Immunization of the Immunocompromised Host
Clin. Microbiol. Rev., January 1, 1998; 11(1): 1 - 26.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1987 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1987 by The American Association of Immunologists, Inc. All rights reserved.