The Journal of Immunology, Vol 138, Issue 10 3284-3289, Copyright © 1987 by American Association of Immunologists

ARTICLES

Production of interleukin 1 by adult T cell leukemia (ATL) cell lines

U Yamashita, F Shirakawa and H Nakamura

The accessory function for T cell activation and the production of interleukin 1 (IL 1) of adult T cell leukemia (ATL) cell lines were studied in vitro. ATL cell lines such as Hut-102, MT-1, and MT-2 functioned as accessory cells for the stimulation of human T cell proliferative response induced with concanavalin A (Con A) and induced allogeneic mixed lymphocyte reaction. Cell lysates of three ATL cell lines and the culture supernatant of MT-2 cells had activities to stimulate murine thymocyte proliferative response. Then we studied physicochemical properties of the factors produced by MT-2 cells. The m.w. of the factors were approximately 15,000 by Sephacryl S-200 column chromatography, and their isoelectric point values were 5.4 and 4.8 by chromatofocussing technique. No fraction contained interleukin 2 (IL 2) activities to stimulate IL 2-dependent murine cytotoxic T cell line. The thymocyte-stimulating activities of the factors were absorbed with rabbit anti-IL 1 alpha antiserum, but not with anti-IL 1 beta antiserum. Furthermore, messenger RNA extracted from MT-2 cells hybridized to complementary DNA of IL 1 alpha, but not of IL 1 beta, by Northern blot hybridization analysis. The factors from MT-2 cells could stimulate the production of IL 2 and the expression of IL 2 receptors of human T cells in the presence of Con A as well as recombinant IL 1 alpha and IL 1 beta did, and these activities were also blocked by rabbit anti-IL 1 alpha antiserum, but not by anti-IL 1 beta antiserum. These results suggest that the factors produced by MT-2 cells correspond to IL 1 alpha. However, the accessory function of MT-2 cells for T cell activation was not blocked by rabbit anti-IL 1 antiserum. These results suggest that ATL cell lines produce IL 1-like factors, but the accessory function of ATL cell lines for T cell activation is mediated by some other mechanisms rather than by secreted IL 1-like factors.

This article has been cited by other articles:

				Y. Niki, H. Yamada, T. Kikuchi, Y. Toyama, H. Matsumoto, K. Fujikawa, and N. Tada Membrane-Associated IL-1 Contributes to Chronic Synovitis and Cartilage Destruction in Human IL-1{alpha} Transgenic Mice J. Immunol., January 1, 2004; 172(1): 577 - 584. [Abstract] [Full Text] [PDF]

				Y.-T. Huang, T.-S. Sheen, C.-L. Chen, J. Lu, Y. Chang, J.-Y. Chen, and C.-H. Tsai Profile of Cytokine Expression in Nasopharyngeal Carcinomas: A Distinct Expression of Interleukin 1 in Tumor and CD4+ T Cells Cancer Res., April 1, 1999; 59(7): 1599 - 1605. [Abstract] [Full Text] [PDF]

				J. Wright, K. Gunter, H Mitsuya, S. Irving, K Kelly, and U Siebenlist Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells Science, May 4, 1990; 248(4955): 588 - 591. [Abstract] [PDF]