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The Journal of Immunology, Vol 137, Issue 4 1264-1269, Copyright © 1986 by American Association of Immunologists

ARTICLES

Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten

A Sonnenberg, P van Balen, T Hengeveld, GJ Kolvenbag, RP Van Hoeven and J Hilgers

Two hybridomas, derived by fusing mouse myeloma cells with spleen cells from a rat immunized with mouse mammary tumors, have been shown to produce antibodies that recognize cell surface antigens on mesenchymal cells in a variety of tissues. Evidence presented in this report suggests that these antibodies detect overlapping epitopes on the Forssman glycolipid hapten (GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1- 4Gal beta 1-4Glc beta 1-1Cer). One antibody (33B12) reacts with the terminal sugar sequence GalNAc alpha 1-3GalNAc and is specific for Forssman. The other antibody (117C9) recognizes the internal sugar sequence GalNAc beta 1-3Gal. The terminal sugar sequence GalNAc beta 1- 3Gal in globoside, as well as the internal sugar sequence GalNAc beta 1- 4Gal in asialo-GM1, is not recognized as an antigenic determinant by 117C9. Nevertheless, the 117C9 antibody does not react exclusively with the Forssman antigen. In a lipid extract fractionated by Folch partition of mouse mammary tumors, the antibody also detects other glycolipids.


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A Unique Thymic Fibroblast Population Revealed by the Monoclonal Antibody MTS-15
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