| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 137, Issue 3 819-825, Copyright © 1986 by American Association of Immunologists
ARTICLES |
A Weiss, B Manger and J Imboden
In addition to the T3/antigen receptor complex (T3/Ti), other T cell surface molecules participate in early events involved in human T cell activation. In this report we document that monoclonal antibody 9.3, which recognizes a 90,000 dalton homodimer expressed on human T cells, synergizes with ligands reacting with T3/Ti to activate purified T cells and Jurkat, a human T cell leukemic line. Unlike phorbol myristate acetate (PMA), 9.3 was able to synergize only with anti-T3 or anti-Ti if these antibodies were immobilized. Moreover, 9.3 failed to synergize with the calcium ionophore ionomycin. At high concentrations only, 9.3 could synergize with PMA in the activation of Jurkat and a T3/Ti negative mutant of Jurkat. At such high concentrations of 9.3, small transient increases in cytoplasmic free calcium ((Ca++)i) were detected in quin 2-loaded Jurkat cells. This increase in (Ca++)i was the result of release of internal stores of calcium. 9.3 induced the hydrolysis of polyphosphoinositides, albeit the magnitude of inositol phosphates generated in response to 9.3 was substantially less than that observed with anti-Ti. No effect on pkC translocation was observed in Jurkat cells stimulated with 9.3. Although the small increase in (Ca++)i induced by 9.3 may account for its synergy with PMA, this effect is unlikely to account for the more potent synergistic effect observed with 9.3 and phytohemagglutinin or immobilized anti-T3 and anti-Ti antibodies.
This article has been cited by other articles:
|
|
 |
|
  O. D. Schneider, A. A. Weiss, and W. E. Miller Pertussis Toxin Utilizes Proximal Components of the T-Cell Receptor Complex To Initiate Signal Transduction Events in T Cells Infect. Immun., August 1, 2007; 75(8): 4040 - 4049. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sun, K. T. Ames, I. Suzuki, and P. J. Fink The Cytoplasmic Domain of Fas Ligand Costimulates TCR Signals J. Immunol., August 1, 2006; 177(3): 1481 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bernard, B. Riteau, J. D. Hansen, R. B. Phillips, F. Michel, P. Boudinot, and A. Benmansour Costimulatory Receptors in a Teleost Fish: Typical CD28, Elusive CTLA4 J. Immunol., April 1, 2006; 176(7): 4191 - 4200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-B. Wang, B.-F. Zhao, Q. Zhao, J.-H. Piao, J. Liu, Q. Lin, and H.-L. Huang A New Recombinant Single Chain Trispecific Antibody Recruits T Lymphocytes to Kill CEA (Carcinoma Embryonic Antigen) Positive Tumor Cells In Vitro Efficiently J. Biochem., April 1, 2004; 135(4): 555 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ishii, K. Ohnuma, A. Murakami, N. Takasawa, T. Yamochi, S. Iwata, M. Uchiyama, N. H. Dang, H. Tanaka, and C. Morimoto SS-A/Ro52, an Autoantigen Involved in CD28-Mediated IL-2 Production J. Immunol., April 1, 2003; 170(7): 3653 - 3661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Tatari-Calderone, R. T. Semnani, T. B. Nutman, J. Schlom, and H. Sabzevari Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction J. Immunol., December 1, 2002; 169(11): 6162 - 6169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Heinly, G. D. Sempowski, D. M. Lee, D. D. Patel, P. M. McDermott, R. M. Scearce, C. B. Thompson, and B. F. Haynes Comparison of thymocyte development and cytokine production in CD7-deficient, CD28-deficient and CD7/CD28 double-deficient mice Int. Immunol., February 1, 2001; 13(2): 157 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Witherden, R. Boismenu, and W. L. Havran CD81 and CD28 Costimulate T Cells Through Distinct Pathways J. Immunol., August 15, 2000; 165(4): 1902 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Leung, L. Haughn, A. Veillette, R. G. Hawley, R. Rottapel, and M. Julius TCR{alpha}{beta}-Independent CD28 Signaling and Costimulation Require Non-CD4-Associated Lck J. Immunol., August 1, 1999; 163(3): 1334 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Siefken, S. Klein-He{beta}ling, E. Serfling, R. Kurrle, and R. Schwinzer A CD28-Associated Signaling Pathway Leading to Cytokine Gene Transcription and T Cell Proliferation Without TCR Engagement J. Immunol., August 15, 1998; 161(4): 1645 - 1651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Okkenhaug and R. Rottapel Grb2 Forms an Inducible Protein Complex with CD28 through a Src Homology 3 Domain-Proline Interaction J. Biol. Chem., August 14, 1998; 273(33): 21194 - 21202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. C. Liao, S. Fournier, N. Killeen, A. Weiss, J. P. Allison, and D. R. Littman Itk Negatively Regulates Induction of T Cell Proliferation by CD28 Costimulation J. Exp. Med., July 21, 1997; 186(2): 221 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fraser, B. Irving, G. Crabtree, and A Weiss Regulation of interleukin-2 gene enhancer activity by the T cell accessory molecule CD28 Science, January 18, 1991; 251(4991): 313 - 316. [Abstract] [PDF]
|
|
|
|
|
|
S. Bounou, N. Dumais, and M. J. Tremblay Attachment of Human Immunodeficiency Virus-1 (HIV-1) Particles Bearing Host-encoded B7-2 Proteins Leads to Nuclear Factor-kappa B- and Nuclear Factor of Activated T Cells-dependent Activation of HIV-1 Long Terminal Repeat Transcription J. Biol. Chem., February 23, 2001; 276(9): 6359 - 6369. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
