The Journal of Immunology, Vol 136, Issue 7 2551-2555, Copyright © 1986 by American Association of Immunologists

ARTICLES

Cleaved forms of C-reactive protein are associated with platelet inhibition

BA Fiedel and H Gewurz

C-reactive protein (CRP) is the prototypic acute phase reactant and serves clinically as a marker of inflammation and tissue destruction. When native CRP pentamer was incubated with Streptomyces griseus protease, a newly formed and transient ability to inhibit platelet aggregation stimulated by adenosine diphosphate or collagen was often elicited early during the course of enzymatic digestion. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses of the digests revealed that platelet inhibitory activity correlated with altered electrophoretic mobility and reductions in subunit and pentameric m.w. Minimally degraded forms of CRP were also isolated "de novo" from inflammatory fluids and, like their enzyme degraded counterparts, inhibited platelet activation. Dissociation of degraded CRP with SDS followed by the removal of SDS resulted in the separation fragments which inhibited platelet function. We propose that in a degradative environment, such as at sites of inflammation/tissue damage or through the action of serum proteases, CRP may transitorily down-regulate the platelet.