The Journal of Immunology, Vol 136, Issue 3 892-897, Copyright © 1986 by American Association of Immunologists

ARTICLES

Specificity analysis of human anti-DNA antibodies

S Hoch and J Schwaber

Human hybrids producing anti-DNA antibodies were generated by the fusion of pokeweed mitogen-stimulated splenic lymphocytes from a child with sickle cell anemia to GM4672. Of 19 hybrids, three (15%) produced anti-DNA antibody as detected by an enzyme linked immunosorbent assay. One subclone from each of these three hybrids was then characterized. All produced IgM antibody in large amounts ranging from 22 to 266 micrograms/ml per million cells per 24 hr. All three antibodies bound both double- and single-stranded DNA. Competitive inhibition assays revealed the greatest inhibition of DNA binding with the ribohomopolymers polyinosinic and polyguanylic acid. A complex pattern of cross-reactivity with various other polynucleotides and with some phospholipids was observed. Subtle differences were found among the three antibodies in light chain class and some of the binding specificities. By using a modified Farr assay, all three monoclonals were found to be of low to intermediate affinity. These results confirm that anti-DNA antibodies apparently equivalent to those seen in patients with SLE can be derived from "normal" nonautoimmune individuals.

This article has been cited by other articles:

				K Haugbro, J C Nossent, T Winkler, Y Figenschau, and O P Rekvig Anti-dsDNA antibodies and disease classification in antinuclear antibody positive patients: the role of analytical diversity Ann Rheum Dis, April 1, 2004; 63(4): 386 - 394. [Abstract] [Full Text] [PDF]

				R. Suenaga and N. I. Abdou Lupus-Derived Human Monoclonal IgM anti-DNA Antibody Displays Monospecificity, High Affinity and Private Idiotype Specificity Lupus, December 1, 1992; 1(6): 363 - 368. [Abstract] [PDF]