The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grabstein, K.
Right arrow Articles by Larsen, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grabstein, K.
Right arrow Articles by Larsen, A.

The Journal of Immunology, Vol 136, Issue 12 4503-4508, Copyright © 1986 by American Association of Immunologists

ARTICLES

Expression of interleukin 2, interferon-gamma, and the IL 2 receptor by human peripheral blood lymphocytes

K Grabstein, S Dower, S Gillis, D Urdal and A Larsen

Human peripheral blood T lymphocytes were treated with recombinant interleukin 2, mitogens, and dexamethasone. The resulting accumulation of mRNA for interleukin 2 (IL 2), the interleukin 2 receptor (IL 2R), and interferon-gamma (IFN-gamma) was measured. IL 2 was found to regulate the levels of each of these mRNA. The expression of mRNA for IL 2, IL 2R, and IFN-gamma correlated very well with the levels of protein observed. In populations of peripheral blood T lymphocytes, the production of IL 2 and IFN-gamma were not necessarily coordinately expressed. The sequential expression of these mRNA was investigated in order to determine whether they might be independent of the action of IL 2. IFN-gamma and IL 2 mRNA showed biphasic accumulations. IL 2 mRNA accumulated very rapidly, within 60 min after mitogen stimulation and before any detectable IL 2R mRNA accumulation. Similarly, IFN-gamma mRNA accumulated rapidly, simultaneously with IL 2 mRNA. This early peak of IFN-gamma mRNA, therefore, is likely to be independent of IL 2 action. Both IL 2 and IFN-gamma mRNA then showed later peak times of accumulation. IL 2 mRNA levels peaked at 5 hr after mitogen stimulation, whereas IFN-gamma mRNA levels peaked at 20 hr. IL 2R mRNA continued to accumulate for the full 40 hr of these kinetic experiments. The later accumulations of IFN-gamma and IL 2R mRNA and the resulting expression of the corresponding proteins may therefore be dependent on the earlier production of IL 2 and its subsequent interaction with the IL 2R on the surface of such activated T cells.


This article has been cited by other articles:


Home page
 IOVSHome page
A. Kato, H. Kimura, K. Okabe, J. Okabe, N. Kunou, and Y. Ogura
Feasibility of Drug Delivery to the Posterior Pole of the Rabbit Eye with an Episcleral Implant
Invest. Ophthalmol. Vis. Sci., January 1, 2004; 45(1): 238 - 244.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Okabe, H. Kimura, J. Okabe, A. Kato, N. Kunou, and Y. Ogura
Intraocular Tissue Distribution of Betamethasone after Intrascleral Administration Using a Non-biodegradable Sustained Drug Delivery Device
Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2702 - 2707.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. Okabe, H. Kimura, N. Kunou, K. Okabe, A. Kato, and Y. Ogura
Biodegradable Intrascleral Implant for Sustained Intraocular Delivery of Betamethasone Phosphate
Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 740 - 744.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. R. McFadden Jr.
Inhaled Glucocorticoids and Acute Asthma . Therapeutic Breakthrough or Nonspecific Effect?
Am. J. Respir. Crit. Care Med., March 1, 1998; 157(3): 677 - 678.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1986 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1986 by The American Association of Immunologists, Inc. All rights reserved.