The Journal of Immunology, Vol 135, Issue 2 879-885, Copyright © 1985 by American Association of Immunologists

ARTICLES

Undiminished immunologic tolerance to contact sensitivity in mast cell- deficient W/Wv and Sl/Sld mice

YA Mekori and SJ Galli

Several mast cell-derived mediators, when tested individually, have actions that may be considered immunosuppressive or anti-inflammatory. Yet evidence concerning the importance of mast cells to the down regulation of T cell-dependent immune responses in vivo is scanty and contradictory. To test directly the net contribution of intact mast cells to the suppression of delayed hypersensitivity reactions in vivo, we attempted to elicit tolerance to contact sensitivity in W/Wv or Sl/Sld mast cell-deficient mice, and compared their responses with those of littermate control mice with normal numbers of mast cells. By using three different measures of delayed hypersensitivity (ear swelling, weight ratios of challenged and control ears, and 125I-IUDR- labeled leukocyte infiltration into challenged and control ears), we detected no deficiency in the 24 hr contact sensitivity reactions to DNFB in control (nontolerized) W/Wv or Sl/Sld mice. We thus confirmed previous work indicating that mast cells are not essential for the induction and elicitation of delayed hypersensitivity. Furthermore, mast cell-deficient and control mice developed equivalent levels of tolerance to contact sensitivity. This was true for tolerance induced by DNBS administered i.v. 7 days before epicutaneous sensitization with DNFB, and for tolerance induced by supraoptimal sensitization with DNFB. W/Wv and Sl/Sld mice also served as suitable donors and recipients for putative suppressor T cells (Ts) induced by i.v. DNBS. We conclude that mast cells make little or no contribution to the modulation of Ts activity in two different models of Ts-dependent tolerance to contact sensitivity.

This article has been cited by other articles:

				M. U. Norman, J. Hwang, S. Hulliger, C. S. Bonder, J. Yamanouchi, P. Santamaria, and P. Kubes Mast Cells Regulate the Magnitude and the Cytokine Microenvironment of the Contact Hypersensitivity Response Am. J. Pathol., June 1, 2008; 172(6): 1638 - 1649. [Abstract] [Full Text] [PDF]

				D. M. Jawdat, G. Rowden, and J. S. Marshall Mast Cells Have a Pivotal Role in TNF-Independent Lymph Node Hypertrophy and the Mobilization of Langerhans Cells in Response to Bacterial Peptidoglycan J. Immunol., August 1, 2006; 177(3): 1755 - 1762. [Abstract] [Full Text] [PDF]

				H. Suto, S. Nakae, M. Kakurai, J. D. Sedgwick, M. Tsai, and S. J. Galli Mast Cell-Associated TNF Promotes Dendritic Cell Migration J. Immunol., April 1, 2006; 176(7): 4102 - 4112. [Abstract] [Full Text] [PDF]

				N. Tedla, H.-W. Wang, H. P. McNeil, N. Di Girolamo, T. Hampartzoumian, D. Wakefield, and A. Lloyd Regulation of T Lymphocyte Trafficking into Lymph Nodes During an Immune Response by the Chemokines Macrophage Inflammatory Protein (MIP)-1{alpha} and MIP-1{beta} J. Immunol., November 15, 1998; 161(10): 5663 - 5672. [Abstract] [Full Text] [PDF]