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The Journal of Immunology, Vol 135, Issue 1 117-122, Copyright © 1985 by American Association of Immunologists

ARTICLES

Induction of high affinity IL 2 receptors on B cells responding to anti- Ig and T cell-derived helper factors

S Prakash, RJ Robb, RD Stout and DC Parker

We have reported that IL 2 is one of the essential helper factors in culture supernatants from concanavalin A-activated spleen cells or T cell hybridomas that support proliferation and immunoglobulin secretion in B cell cultures responding to anti-immunoglobulin. Here we show that cells in such cultures consume IL 2 and bear high affinity IL 2 receptors detected by binding of purified, radiolabeled IL 2. Induction of high affinity IL 2 receptors depends on addition of both anti- immunoglobulin and helper factors, and does not occur in cultures given only anti-Ig, only helper factors, concanavalin A plus helper factors, or LPS. The majority of IL 2 receptors are on cells that also bear endogenous membrane immunoglobulin, because they are found in the membrane immunoglobulin-positive fraction when cultured cells are separated by fluorescence sorting after overnight culture in the absence of anti-immunoglobulin to allow reexpression of membrane immunoglobulin.


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C. Johnson-Leger, J. R. Christenson, M. Holman, and G. G. B. Klaus
Evidence for a Critical Role for IL-2 in CD40-Mediated Activation of Naive B Cells by Primary CD4 T Cells
J. Immunol., November 1, 1998; 161(9): 4618 - 4626.
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