The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, R. A.
Right arrow Articles by Stutman, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, R. A.
Right arrow Articles by Stutman, O.

The Journal of Immunology, Vol 133, Issue 6 2925-2932, Copyright © 1984 by American Association of Immunologists

ARTICLES

T cell repopulation from functionally restricted splenic progenitors: 10,000-fold expansion documented by using limiting dilution analyses

RA Miller and O Stutman

Mice depleted of T cells by thymectomy, lethal irradiation, and reconstitution with Thy-1-depleted syngeneic bone marrow were given graded doses of splenic T cells to see whether post-thymic cells had the ability to regenerate immune function in these hosts. Using limiting dilution methods to estimate the number of antigen- and mitogen-responsive cells in recipients 12 to 20 wk after reconstitution, we found that new helper and cytotoxic precursor cells were produced, but attained levels only 10 to 20% of normal. Because these repopulated mice were able to produce nearly normal levels of helper and cytotoxic activity in conventional, high density cultures, despite their relative paucity of precursors, we infer that their normal function in conventional assays may reflect a balanced deficiency of effector and regulatory cell types. Surface phenotyping of the progenitor cells responsible for repopulation showed that Lyt-2- cells were required for helper cell regeneration and that Lyt-2+ cells acted as progenitors only for the cytotoxic lineage, contrary to earlier speculation that the splenic Lyt-1+ 2+ (Ly-123) pool included cells antecedent to both effector lineages. Comparison of the number of injected progenitors needed to produce repopulation with the number of new precursor cells eventually produced suggests that the relevant progenitors are able to undergo 10,000-fold expansion in 12 to 20 wk. Numerical expansion in the periphery from thymic-processed cells could well be a major source of new lymphocytes in adult mice.


This article has been cited by other articles:


Home page
 BloodHome page
K. Rezvani, S. Mielke, M. Ahmadzadeh, Y. Kilical, B. N. Savani, J. Zeilah, K. Keyvanfar, A. Montero, N. Hensel, R. Kurlander, et al.
High donor FOXP3-positive regulatory T-cell (Treg) content is associated with a low risk of GVHD following HLA-matched allogeneic SCT
Blood, August 15, 2006; 108(4): 1291 - 1297.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. D. French, C. L. Roark, W. K. Born, and R. L. O'Brien
{gamma}{delta} T cell homeostasis is established in competition with {alpha}{beta} T cells and NK cells
PNAS, October 11, 2005; 102(41): 14741 - 14746.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Matejuk, C. Hopke, A. A. Vandenbark, P. D. Hurn, and H. Offner
Middle-Age Male Mice Have Increased Severity of Experimental Autoimmune Encephalomyelitis and Are Unresponsive to Testosterone Therapy
J. Immunol., February 15, 2005; 174(4): 2387 - 2395.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
U. Wagner, M. Pierer, M. Wahle, F. Moritz, S. Kaltenhauser, and H. Hantzschel
Ex Vivo Homeostatic Proliferation of CD4+ T Cells in Rheumatoid Arthritis Is Dysregulated and Driven by Membrane-Anchored TNF{alpha}
J. Immunol., August 15, 2004; 173(4): 2825 - 2833.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. R. M. Almeida, N. Legrand, M. Papiernik, and A. A. Freitas
Homeostasis of Peripheral CD4+ T Cells: IL-2R{alpha} and IL-2 Shape a Population of Regulatory Cells That Controls CD4+ T Cell Numbers
J. Immunol., November 1, 2002; 169(9): 4850 - 4860.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. J. Fry and C. L. Mackall
Interleukin-7: from bench to clinic
Blood, May 13, 2002; 99(11): 3892 - 3904.
[Full Text] [PDF]

Home page
 BloodHome page
O. Alpdogan, C. Schmaltz, S. J. Muriglan, B. J. Kappel, M.-A. Perales, J. A. Rotolo, J. A. Halm, B. E. Rich, and M. R. M. van den Brink
Administration of interleukin-7 after allogeneic bone marrow transplantation improves immune reconstitution without aggravating graft-versus-host disease
Blood, October 1, 2001; 98(7): 2256 - 2265.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
A. R.M. Almeida, J. A.M. Borghans, and A. A. Freitas
T Cell Homeostasis: Thymus Regeneration and Peripheral T Cell Restoration in Mice with a Reduced Fraction of Competent Precursors
J. Exp. Med., August 27, 2001; 194(5): 591 - 600.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Malmstrom, D. Shipton, B. Singh, A. Al-Shamkhani, M. J. Puklavec, A. N. Barclay, and F. Powrie
CD134L Expression on Dendritic Cells in the Mesenteric Lymph Nodes Drives Colitis in T Cell-Restored SCID Mice
J. Immunol., June 1, 2001; 166(11): 6972 - 6981.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. J. Fry, B. L. Christensen, K. L. Komschlies, R. E. Gress, and C. L. Mackall
Interleukin-7 restores immunity in athymic T-cell-depleted hosts
Blood, March 15, 2001; 97(6): 1525 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. R. M. Clarke and A. Y. Rudensky
Survival and Homeostatic Proliferation of Naive Peripheral CD4+ T Cells in the Absence of Self Peptide:MHC Complexes
J. Immunol., September 1, 2000; 165(5): 2458 - 2464.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
D. Spaner, X. Sheng-Tanner, K. Raju, B. Rabinovich, H. Messner, and R. G. Miller
Long-term persistence of IL-2-unresponsive allogeneic T cells in sublethally irradiated SCID mice
Int. Immunol., October 1, 1999; 11(10): 1601 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
J. Bender, T. Mitchell, J. Kappler, and P. Marrack
CD4+ T Cell Division in Irradiated Mice Requires Peptides Distinct from Those Responsible for Thymic Selection
J. Exp. Med., August 2, 1999; 190(3): 367 - 374.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. A. Timm and M. L. Thoman
Maturation of CD4+ Lymphocytes in the Aged Microenvironment Results in a Memory-Enriched Population
J. Immunol., January 15, 1999; 162(2): 711 - 717.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. E. Boursalian and K. Bottomly
Stability of Naive and Memory Phenotypes on Resting CD4 T Cells In Vivo
J. Immunol., January 1, 1999; 162(1): 9 - 16.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K.'i. Imanishi, K. Seo, H. Kato, T. Miyoshi-Akiyama, R.-H. Zhang, Y. Takanashi, Y. Imai, and T. Uchiyama
Post-Thymic Maturation of Migrating Human Thymic Single-Positive T Cells: Thymic CD1a- CD4+ T Cells Are More Susceptible to Anergy Induction by Toxic Shock Syndrome Toxin-1 than Cord Blood CD4+ T Cells
J. Immunol., January 1, 1998; 160(1): 112 - 119.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
B Rocha and H von Boehmer
Peripheral selection of the T cell repertoire
Science, March 8, 1991; 251(4998): 1225 - 1228.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1984 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1984 by The American Association of Immunologists, Inc. All rights reserved.