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The Journal of Immunology, Vol 133, Issue 2 758-763, Copyright © 1984 by American Association of Immunologists

ARTICLES

Enhanced production of human gamma-interferon in nylon column- fractionated cell cultures

R van Reis, AS Lubiniecki, RA Olson, RR Stromberg, JA Madsen and LI Friedman

The production of human gamma-interferon (HuIFN-gamma) in unfractionated and nylon wool column-fractionated leukocyte cell cultures stimulated with PMA and PHA was investigated. Production was studied with normal and reduced autologous serum protein levels in 96- hr spinner cultures. A 10- to 15-fold enhancement of production and a 50-fold increase in specific activity of crude HuIFN-gamma was demonstrated in nylon column-fractionated/reduced serum cell cultures. Kinetic analysis revealed a production rate maximum within 6 hr of induction in unprocessed cell cultures, whereas production occurred at an essentially constant rate for 48 hr in fractionated cell cultures.




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