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The Journal of Immunology, Vol 131, Issue 6 3049-3051, Copyright © 1983 by American Association of Immunologists

ARTICLES

Effect of T cell-derived lymphokines containing B cell differentiation factor(s) for IgG (BCDF gamma) on gamma-specific mRNA in murine B cells

S Jones, YW Chen, P Isakson, J Layton, E Pure, C Word, PH Krammer, P Tucker and ES Vitetta

Cytoplasmic RNA was isolated from cells cultured with LPS and LPS plus a T cell-derived supernatant (SN) (PK 7.1) containing B cell differentiation factors. The steady state levels of isotype-specific mRNA were assessed by Northern blot analysis with gamma-specific CH3 probes. It was demonstrated that the SN induces an increase in the level of mRNA for gamma 1 and a concomitant decrease in the levels of mRNA for gamma 2b and gamma 3.


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J. R. Podojil and V. M. Sanders
Selective Regulation of Mature IgG1 Transcription by CD86 and {beta}2-Adrenergic Receptor Stimulation
J. Immunol., May 15, 2003; 170(10): 5143 - 5151.
[Abstract] [Full Text] [PDF]


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