The Journal of Immunology, Vol 131, Issue 5 2135-2140, Copyright © 1983 by American Association of Immunologists

ARTICLES

Response against single minor histocompatibility antigens. I. Functional and immunogenetic analysis of cloned cytolytic T cells

DC Roopenian, MB Widmer, CG Orosz and FH Bach

A clonal approach was used to investigate the cellular basis of a T cell response to single minor histocompatibility antigens (miHA). This analysis was performed by functional and immunogenetic characterization of a large number of clones derived from short-term mixed leukocyte culture (MLC) populations generated against the miHA, H-1.3. Forty-nine clones isolated from such MLC were specifically cytolytic for H-1.3- bearing, H-2Db-compatible target cells. Thirty-seven of the 49 cytolytic clones were driven to proliferate when stimulated by spleen cells bearing the H-1.3 alloantigen in the absence of added T cell- derived growth factor(s) (GF). The remaining 12 clones proliferated only when GF was added. A strong positive correlation was observed between antigen-induced proliferation and the production of interleukin 2 (IL 2) activity. A similar correlation was observed when comparing the ability of both antigen and concanavalin A to induce IL 2 activity from the clones. These data suggest that i) antigen-driven or helper T cell-independent cytolytic T cells (HITc) are frequent components of an MLC response to a single miHA, and ii) the ability of HITc to undergo antigen-driven proliferation is related to their ability to produce antigen-induced GF.

This article has been cited by other articles:

				R. Yadav, Y. Yoshimura, A. Boesteanu, G. J. Christianson, W. U. Ajayi, R. Shashidharamurthy, A. K. Stanic, D. C. Roopenian, and S. Joyce The H4b Minor Histocompatibility Antigen Is Caused by a Combination of Genetically Determined and Posttranslational Modifications J. Immunol., May 15, 2003; 170(10): 5133 - 5142. [Abstract] [Full Text] [PDF]

				E. Y. Choi, Y. Yoshimura, G. J. Christianson, T. J. Sproule, S. Malarkannan, N. Shastri, S. Joyce, and D. C. Roopenian Quantitative Analysis of the Immune Response to Mouse Non-MHC Transplantation Antigens In Vivo: The H60 Histocompatibility Antigen Dominates Over All Others J. Immunol., April 1, 2001; 166(7): 4370 - 4379. [Abstract] [Full Text] [PDF]

				P. A. Eden, G. J. Christianson, P. Fontaine, P. J. Wettstein, C. Perreault, and D. C. Roopenian Biochemical and Immunogenetic Analysis of an Immunodominant Peptide (B6dom1) Encoded by the Classical H7 Minor Histocompatibility Locus J. Immunol., April 15, 1999; 162(8): 4502 - 4510. [Abstract] [Full Text] [PDF]

				A. Boesteanu, M. Brehm, L. M. Mylin, G. J. Christianson, S. S. Tevethia, D. C. Roopenian, and S. Joyce A Molecular Basis for How a Single TCR Interfaces Multiple Ligands J. Immunol., November 1, 1998; 161(9): 4719 - 4727. [Abstract] [Full Text] [PDF]

				S. Malarkannan, P. P. Shih, P. A. Eden, T. Horng, A. R. Zuberi, G. Christianson, D. Roopenian, and N. Shastri The Molecular and Functional Characterization of a Dominant Minor H Antigen, H60 J. Immunol., October 1, 1998; 161(7): 3501 - 3509. [Abstract] [Full Text] [PDF]