The Journal of Immunology, Vol 130, Issue 6 2505-2507, Copyright © 1983 by American Association of Immunologists

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Phorbolester-treated human lymphocytes are susceptible to natural killer cell-mediated cytolysis

D Kabelitz and HG Kunkel

The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) was found in this study to render normal human lymphocytes susceptible to allogeneic and autologous natural killer (NK) cell lysis. Both T and non-T cells became susceptible after culture for 3 days in the presence of 1 ng/ml TPA. Effector cells were nonadherent mononuclear cells of low density, enriched for large granular lymphocytes and HNK-1+ cells. Activation of effector cells with interferon increased lysis of TPA- treated lymphocytes. The present system may provide a new model to study NK cell target structures.

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