The Journal of Immunology, Vol 129, Issue 3 1008-1011, Copyright © 1982 by American Association of Immunologists

ARTICLES

Characterization of a suppressive factor produced by a human T hybridoma

C Grillot-Courvalin, K Dellagi, A Chevalier and JC Brouet

The mode of action and the target cell of a suppressive factor continuously and spontaneously produced by a human hybrid T cell line constructed recently are reported. This factor was shown to suppress pokeweed mitogen-induced B cell differentiation when added within the first 48 hr of culture. It does not suppress B cell differentiation induced by Nocardia extracts, a relatively T-independent mitogen. The target cell for this factor seems to be a T lymphocyte in that it can be absorbed by E-rosetting cells but not by B lymphocytes or monocytes. This factor does not impair mitogen or allogeneic-induced lymphocyte proliferation and has an apparent m.w. of approximately 45,000 as estimated by Sephacryl S200 gel chromatography.

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				J Laurence and L Mayer Immunoregulatory lymphokines of T hybridomas from AIDS patients: constitutive and inducible suppressor factors Science, July 6, 1984; 225(4657): 66 - 69. [Abstract] [PDF]