The Journal of Immunology, Vol 128, Issue 6 2393-2398, Copyright © 1982 by American Association of Immunologists

ARTICLES

Constitutive production of a unique lymphokine (IL 3) by the WEHI-3 cell line

JC Lee, AJ Hapel and JN Ihle

It has been recently demonstrated that conditioned media from Con A- stimulated splenocyte cultures contain a novel lymphokine termed IL 3. IL 3 is characterized by its ability to induce 20-alpha-hydroxysteroid dehydrogenase in spleen cells from young nu/nu mice. In search of a convenient source for biochemical and in vivo studies of this lymphokine, a number of established murine cell lines were screened for the constitutive and induced production of Il 3. It was found that WEHI- 3 cells, originally designated as a myelomonocyte cell line, produced high levels of IL 3 constitutively. Added mitogen and/or phorbol acetate did not enhance the production of IL 3. Production of IL 3 varied among various sublines of WEHI-3. IL 3 purified from WEHI-3- conditioned media has biochemical and biologic characteristics identical to those obtained from Con A-conditioned media. WEHI-3 conditioned media, however, generally contained 100-fold higher levels of IL 3 than conditioned media from Con A-stimulated splenic lymphocytes.

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