The Journal of Immunology, Vol 128, Issue 3 1159-1162, Copyright © 1982 by American Association of Immunologists

ARTICLES

The area of attachment of cytotoxic T lymphocytes to their target cells shows high motility and polarization of actin, but not myosin

JE Ryser, E Rungger-Brandle, C Chaponnier, G Gabbiani and P Vassalli

Conjugates of cytotoxic T lymphocytes attached to their target cells were studied by double immunofluorescence on fixed smears to detect simultaneously the localization of actin and myosin within the cells. Actin was found to be polarized in the area of attachment of the lymphocytes (but not of the target cells), whereas myosin remained evenly distributed in the cytoplasm. When living conjugates were brought to 37 degrees C to induce cytotoxicity, this pattern remained unchanged, but observation by interference reflexion microcinematography revealed a high motility of the lymphocytes in the contact area. This localized motility in the area of attachment associated with a peculiar actin polarization, which has no equivalent in any type of cell contact presently known, could represent a necessary step in the sequence of events leading to target cell killing by cytotoxic T lymphocytes.

This article has been cited by other articles:

				M. H. Shaffer, R. S. Dupree, P. Zhu, I. Saotome, R. F. Schmidt, A. I. McClatchey, B. D. Freedman, and J. K. Burkhardt Ezrin and Moesin Function Together to Promote T Cell Activation J. Immunol., January 15, 2009; 182(2): 1021 - 1032. [Abstract] [Full Text] [PDF]

				N. B. Martin-Cofreces, J. Robles-Valero, J. R. Cabrero, M. Mittelbrunn, M. Gordon-Alonso, C.-H. Sung, B. Alarcon, J. Vazquez, and F. Sanchez-Madrid MTOC translocation modulates IS formation and controls sustained T cell signaling J. Cell Biol., September 9, 2008; 182(5): 951 - 962. [Abstract] [Full Text] [PDF]

				I. Tskvitaria-Fuller, A. L. Rozelle, H. L. Yin, and C. Wulfing Regulation of Sustained Actin Dynamics by the TCR and Costimulation as a Mechanism of Receptor Localization J. Immunol., September 1, 2003; 171(5): 2287 - 2295. [Abstract] [Full Text] [PDF]

				D. Sancho, M. C. Montoya, A. Monjas, M. Gordon-Alonso, T. Katagiri, D. Gil, R. Tejedor, B. Alarcon, and F. Sanchez-Madrid TCR Engagement Induces Proline-Rich Tyrosine Kinase-2 (Pyk2) Translocation to the T Cell-APC Interface Independently of Pyk2 Activity and in an Immunoreceptor Tyrosine-Based Activation Motif-Mediated Fashion J. Immunol., July 1, 2002; 169(1): 292 - 300. [Abstract] [Full Text] [PDF]

				N Foger, R Marhaba, and M Zoller Involvement of CD44 in cytoskeleton rearrangement and raft reorganization in T cells J. Cell Sci., January 3, 2001; 114(6): 1169 - 1178. [Abstract] [PDF]

				M. Krause, A. S. Sechi, M. Konradt, D. Monner, F. B. Gertler, and J. Wehland Fyn-Binding Protein (Fyb)/Slp-76-Associated Protein (Slap), Ena/Vasodilator-Stimulated Phosphoprotein (Vasp) Proteins and the Arp2/3 Complex Link T Cell Receptor (Tcr) Signaling to the Actin Cytoskeleton J. Cell Biol., April 3, 2000; 149(1): 181 - 194. [Abstract] [Full Text] [PDF]

				B. B. HASHEMI, J. E. PENKALA, C. VENS, H. HULS, M. CUBBAGE, and C. F. SAMS T cell activation responses are differentially regulated during clinorotation and in spaceflight FASEB J, November 1, 1999; 13(14): 2071 - 2082. [Abstract] [Full Text]

				X.-Y. Wang, J. R. Ostberg, and E. A. Repasky Effect of Fever-Like Whole-Body Hyperthermia on Lymphocyte Spectrin Distribution, Protein Kinase C Activity, and Uropod Formation J. Immunol., March 15, 1999; 162(6): 3378 - 3387. [Abstract] [Full Text] [PDF]

				C. E. Sedwick, M. M. Morgan, L. Jusino, J. L. Cannon, J. Miller, and J. K. Burkhardt TCR, LFA-1, and CD28 Play Unique and Complementary Roles in Signaling T Cell Cytoskeletal Reorganization J. Immunol., February 1, 1999; 162(3): 1367 - 1375. [Abstract] [Full Text] [PDF]

				T.S. FINCO, D. YABLONSKI, J. LIN, and A. WEISS The Adapter Proteins LAT and SLP-76 Are Required for T-cell Activation Cold Spring Harb Symp Quant Biol, January 1, 1999; 64(0): 265 - 274. [Abstract] [PDF]

				B. Lowin-Kropf, V. S. Shapiro, and A. Weiss Cytoskeletal Polarization of T Cells Is Regulated by an Immunoreceptor Tyrosine-based Activation Motif-dependent Mechanism J. Cell Biol., February 23, 1998; 140(4): 861 - 871. [Abstract] [Full Text] [PDF]

				R.W. Mays, W.J. Nelson, and J.A. Marrs Generation of Epithelial Cell Polarity: Roles for Protein Trafficking, Membrane-Cytoskeleton, and E-Cadherin-mediated Cell Adhesion Cold Spring Harb Symp Quant Biol, January 1, 1995; 60(0): 763 - 773. [Abstract] [PDF]

				K. Sung, L. Sung, M Crimmins, S. Burakoff, and S Chien Determination of junction avidity of cytolytic T cell and target cell Science, December 12, 1986; 234(4782): 1405 - 1408. [Abstract] [PDF]