The Journal of Immunology, Vol 128, Issue 2 758-767, Copyright © 1982 by American Association of Immunologists

ARTICLES

Cellular and molecular properties of an antigen-specific cytotoxic T lymphocyte line

RC Bleackley, C Havele and V Paetkau

Two cytotoxic T cell lines of CBA/J mouse origin have been generated, cloned, and characterized. One of these, MTL2, is cytotoxic for cells carrying the H-2(d) haplotype, the other MTL3, for H-2(b). Two clones of MTL2 differ significantly from each other in the morphology of the cells, although not in cytotoxicity. One has a higher number of intracellular vacuoles, or vesicles, and shows more of the typical attaching structures to the growth surface ("spikes") compared with the other. Cross-reactivity with a third-party alloantigen is enhanced by adding Con A to the cytotoxicity assay, but there is no self-killing either with or without Con A. The T cell lines show no rearrangement in their immunoglobulin heavy chain J and C(mu) genes. Normal T cells are also not rearranged, apparently, but normal B lymphocytes have both of their heavy chain alleles altered. Finally, cloned MTL2 cells contain less than five molecules of mu (IgM heavy chain constant region) mRNA per cell.

This article has been cited by other articles:

				C. K. Babichuk and R. C. Bleackley Mutational Analysis of the Murine Granzyme B Gene Promoter in Primary T Cells and a T Cell Clone J. Biol. Chem., July 25, 1997; 272(30): 18564 - 18571. [Abstract] [Full Text] [PDF]

				C. K. Babichuk, B. L. Duggan, and R. C. Bleackley In Vivo Regulation of Murine Granzyme B Gene Transcription in Activated Primary T Cells J. Biol. Chem., July 12, 1996; 271(28): 16485 - 16493. [Abstract] [Full Text] [PDF]

				J. Elliott, Y Lin, S. Mizel, R. Bleackley, D. Harnish, and V Paetkau Induction of interleukin 2 messenger RNA inhibited by cyclosporin A Science, December 21, 1984; 226(4681): 1439 - 1441. [Abstract] [PDF]