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The Journal of Immunology, 1980, 124: 2625-2631.
Copyright © 1980 by The American Association of Immunologists, Inc.

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The Journal of Immunology, Vol 124, Issue 6 2625-2631, Copyright © 1980 by American Association of Immunologists


ARTICLES

The rabbit properdin system: I. Identification of a new factor and purification of rabbit properdin

GB Naff, SW Hedrick, WD Ratnoff, MA Novak, MC Smith, C Steiner and EW Todd

Pure preparations of rabbit properdin were obtained from rabbit serum by ion-exchange chromatography. These preparations functioned as properdin when they were measured with the zymosan assay or with a serum reagent selectively depleted of properdin by a specific immunoabsorbent. Properdin in these preparations was in its activated state. A new serum factor was required to measure properdin activity when purified preparations of rabbit properdin were tested with the zymosan assay. This factor was designated as ZBP, or zymosan-binding protein. ZBP appeared to be distinct from known components of the alternative complement pathway and the classical complement system, and it did not appear to be an immunoglobulin.





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